Induction of neutralizing antibodies against HTLV-I envelope proteins after combined genetic and protein immunizations in mice

被引：12

作者：

Grange, MP ^{[1
]}

Armand, MA ^{[1
]}

Audoly, G ^{[1
]}

Thollot, D ^{[1
]}

Desgranges, C ^{[1
]}

机构：

[1] INSERM, U271, F-69424 Lyon 03, France

来源：

DNA AND CELL BIOLOGY | 1997年 / 16卷 / 12期

关键词：

D O I：

10.1089/dna.1997.16.1439

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct DNA inoculation can induce both protective humoral and cellular responses against several viruses. The HTLV-I envelope glycoproteins are the major antigens recognized by sera of HTLV-I infected patients that generate neutralizing immune responses in vitro and in vivo. We compared immune responses elicited after a single inoculation of two plasmids encoding the complete HTLV-I envelope proteins followed or not by gp62 Baculovirus recombinant protein boosts in BALB/c mice. First, we observe that the coexpression of env and rex genes is not sufficient to raise a detectable specific humoral response after a single DNA inoculation, Protein boosts generated a high antibody response in mice primed with DNA expressing HTLV-I envelope proteins as compared to naive and negative central vector primed groups. This humoral response presented high neutralizing antibody titers. These results suggest that a single inoculation of DNA expressing HTLV-I env gene can stimulate memory B-cell clones that are able to respond effectively to subsequent encounters with HTLV-I envelope proteins and a specific cellular T helper cell response in mice.

引用

页码：1439 / 1448

页数：10

共 53 条

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