Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property

被引：148

作者：

Shimizu, Satoshi ^{[1
]}

Kitada, Masaaki ^{[1
]}

Ishikawa, Hiroto ^{[1
]}

Itokazu, Yutaka ^{[1
]}

Wakao, Shohei ^{[1
]}

Dezawa, Mari ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Neurobiol & Anat, Sakyo Ku, Kyoto 6068501, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 359卷 / 04期

关键词：

cell therapy; human mesenchymal cells; transdifferentiation; Schwann cells; transplantation; peripheral nerve injury; reconstruction;

D O I：

10.1016/j.bbrc.2007.05.212

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the availability of human bone marrow stromal cells (MSCs) as a source of transplantation therapy in nerve injury. Human MSCs were subjected to a series of treatments with a reducing agent, retinoic acid and a combination of trophic factors. Morphologically and immunocytochemically, such treated cells differentiated into Schwann cell characteristics in vitro. Cells were filled into a transpermeable tube, transplanted into the gap made in the rat sciatic nerve of a rat and followed up to 3 weeks under the control of immunosuppressant. In contrast to untreated human MSCs, differentiated human MSCs expressed Schwann cell markers in vivo and supported regenerating axons. These results suggest that human MSCs can be induced to be a substitute for Schwann cells that may be applied for nerve regeneration. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：915 / 920

页数：6

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