Biofunctionalization of gold nanoparticles and their spectral properties

被引：20

作者：

Genevieve, Mike

Vieu, Christophe

Carles, Robert

Zwick, Antoine

Briere, Guillaume

Salome, Laurence

Trevisiol, Emmanuelle

机构：

[1] Univ Toulouse, LAAS, CNRS, F-31077 Toulouse 4, France

[2] UPS, LPST, UMR 5477, CNRS,IRSAMC, F-31062 Toulouse 4, France

[3] CNRS, IPBS, F-31077 Toulouse 4, France

[4] INRA, Biochips Platform Genopole Toulouse, UMR 5504, CNRS, F-31077 Toulouse 4, France

[5] INRA, Lab Biotechnol & Bioprocedes, UMR 5504, CNRS, F-31077 Toulouse 4, France

来源：

MICROELECTRONIC ENGINEERING | 2007年 / 84卷 / 5-8期

关键词：

biofunctionalization; Raman spectroscopy; gold nanoparticles; streptavidin; photoluminescence;

D O I：

10.1016/j.mee.2007.01.247

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A simple chemical procedure for fixing streptavidin molecules on the periphery of commercial Au nanoparticles stabilized by citrate groups is presented. Using a flocculation test, we determine the adequate quantity of streptavidin molecules necessary for coating Au nanoparticles with approximately one monolayer of streptavidin. Our results indicate that on average, one molecule of streptavidin occupies an area of 40 nm(2) on the surface of the Au nanoparticles, which is the good order of magnitude with respect to the size of this biomolecule. The optical properties of these nanoparticles were found to be sensitive to the biofunctionalization process. We have observed that the changes in photoluminescence spectra could be used to monitor the coverage ratio of the nanoparticles with streptavidin. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1710 / 1713

页数：4