Neural hyperplasia induced by RNA interference with m4/mα gene activity

被引：7

作者：

Nagel, AC

Apidianakis, Y

Wech, I

Maier, D

Delidakis, C

Preiss, A ^{[1
]}

机构：

[1] Univ Hohenheim, Inst Genet 240, D-70593 Stuttgart, Germany

[2] Inst Mol Biol & Biotechnol, FORTH, GR-71110 Heraklion, Greece

[3] Univ Crete, Dept Biol, GR-71110 Heraklion, Greece

来源：

MECHANISMS OF DEVELOPMENT | 2000年 / 98卷 / 1-2期

关键词：

Barbu; bbu; Bearded; Brd; Brd-C; bristle; dominant-negative; double-strand RNA; enhancer of split; E(spl); E(spl)-C; gain of function; gene family; lateral specification; loss of function; m alpha; m4; mechanosensory organ; neurogenesis; Notch; RNA interference; RNAi; sensory organ precursor; twin of m4; tom;

D O I：

10.1016/S0925-4773(00)00446-9

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The E(spl) complex (E(spl)-C) contains three different classes of genes that are downstream of Notch signaling. The bHLH genes mediate the Notch signal by repressing proneural gene activity, for example during the singularization of mechanosensory organ precursor cells (SOPs). Genes of the second class, the E(spl) m4/m alpha family, antagonize this process if overexpressed. Here we show that this is based on dominant-negative effects since RNA interference gives neurogenic phenotypes indistinguishable from E(spl)-C mutations. Furthermore, a third member of the m4/m alpha gene family, named bbu/tom, behaves differently with respect to RNA expression patterns, its regulation by Notch signaling and loss of function phenotypes. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：19 / 28

页数：10

共 39 条

[1] Overexpression of the m4 and mα genes of the E(spl)-Complex antagonizes Notch mediated lateral inhibition [J].