Spinning-disk confocal microscopy - A cutting-edge tool for imaging of membrane traffic

被引：159

作者：

Nakano, A ^{[1
]}

机构：

[1] RIKEN, Discovery Res Inst, Mol Membrane Biol Lab, Wako, Saitama 3510198, Japan

来源：

CELL STRUCTURE AND FUNCTION | 2002年 / 27卷 / 05期

关键词：

membrane traffic; yeast Golgi apparatus; COPI vesicles; green fluorescent protein; Nipkow-disk confocal scanner; HARP camera;

D O I：

10.1247/csf.27.349

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.

引用

页码：349 / 355

页数：7

共 15 条

[1] A monomeric red fluorescent protein [J].

Campbell, RE ;

Tour, O ;

Palmer, AE ;

Steinbach, PA ;

Baird, GS ;

Zacharias, DA ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7877-7882

[2] Spectral imaging fluorescence microscopy [J].

Haraguchi, T ;

Shimi, T ;

Koujin, T ;

Hashiguchi, N ;

Hiraoka, Y .

GENES TO CELLS, 2002, 7 (09) :881-887

[3]

Ichihara A., 1996, BIOIMAGES, V4, P57

[4]

INOUE S, 2002, METHODS CELL BIOL, V70

[5] A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications [J].

Nagai, T ;

Ibata, K ;

Park, ES ;

Kubota, M ;

Mikoshiba, K ;

Miyawaki, A .

NATURE BIOTECHNOLOGY, 2002, 20 (01) :87-90

[6]

NAKANO A, 2002, PROGR BIOTECHNOLOGY, V22, P45

[7] IDENTIFICATION OF A GENE REQUIRED FOR MEMBRANE-PROTEIN RETENTION IN THE EARLY SECRETORY PATHWAY [J].

NISHIKAWA, S ;

NAKANO, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8179-8183

[8] The debate about transport in the Golgi - Two sides of the same coin? [J].

Pelham, HRB ;

Rothman, JE .

CELL, 2000, 102 (06) :713-719

[9] MEMBRANE-PROTEIN RETRIEVAL FROM THE GOLGI-APPARATUS TO THE ENDOPLASMIC-RETICULUM (ER) - CHARACTERIZATION OF THE RER1 GENE-PRODUCT AS A COMPONENT INVOLVED IN ER LOCALIZATION OF SEC12P [J].

SATO, K ;

NISHIKAWA, S ;

NAKANO, A .

MOLECULAR BIOLOGY OF THE CELL, 1995, 6 (11) :1459-1477

[10] Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins [J].

Sato, K ;

Sato, M ;

Nakano, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (18) :9693-9698

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