Comparison of two DNA extractions and nested PCR, real-time PCR, a new commercial PCR assay, and bacterial culture for detection of Mycobacterium avium subsp paratuberculosis in bovine feces

被引:31
作者
Christopher-Hennings, J [1 ]
Dammen, MA
Weeks, SR
Epperson, WB
Singh, SN
Steinlicht, GL
Fang, Y
Skaare, JL
Larsen, JL
Payeur, JB
Nelson, EA
机构
[1] S Dakota State Univ, Anim Dis Res & Diagnost Lab, Brookings, SD 57007 USA
[2] Murray State Univ, Breathitt Vet Ctr, Hopkinsville, KY 42241 USA
[3] Natl Vet Serv Labs, Ames, IA 50014 USA
关键词
D O I
10.1177/104063870301500201
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In this study, 5 combinations of 2 DNA extractions and 3 polymerase chain reaction (PCR) techniques were compared with culture for the detection of Mycobacterium paratuberculosis directly from bovine feces. These combinations included a new commercial extraction technique combined with a commercial PCR/Southern blot technique, nested PCR (nPCR), or real-time PCR, and a university-developed extraction combined with nPCR or real-time PCR. Four of the 5 combinations had statistically similar sensitivities between 93% and 100% and specificity between 95% and 100%, when compared with culture results from 63 bovine fecal samples. These results indicated that using a commercial extraction with a commercial PCR/Southern blot, nPCR, or real-time PCR, or a university-developed extraction with real-time PCR would result in similar sensitivities to culture for the identification of M. paratuberculosis from bovine feces and are valid alternatives to culture.
引用
收藏
页码:87 / 93
页数:7
相关论文
共 36 条
[1]  
Abe Al-Soud W, 2000, J CLIN MICROBIOL, V38, P4463
[2]   Molecular characterization of Mycobacterium paratuberculosis isolates from sheep, goats, and cattle by hybridization with a DNA probe to insertion element IS900 [J].
Bauerfeind, R ;
Benazzi, S ;
Weiss, R ;
Schliesser, T ;
Willems, H ;
Baljer, G .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (07) :1617-1621
[4]   DETECTION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN BOAR SEMEN BY PCR [J].
CHRISTOPHERHENNINGS, J ;
NELSON, EA ;
NELSON, JK ;
HINES, RJ ;
SWENSON, SL ;
HILL, HT ;
ZIMMERMAN, JJ ;
KATZ, JB ;
YAEGER, MJ ;
CHASE, CCL ;
BENFIELD, DA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (07) :1730-1734
[5]   COMPARISON OF POLYMERASE CHAIN-REACTION TESTS AND FECAL CULTURE FOR DETECTING MYCOBACTERIUM-PARATUBERCULOSIS IN BOVINE FECES [J].
COLLINS, DM ;
STEPHENS, DM ;
DELISLE, GW .
VETERINARY MICROBIOLOGY, 1993, 36 (3-4) :289-299
[6]  
COLLINS DM, 1989, FEMS MICROBIOL LETT, V60, P165
[7]   Diagnosis of paratuberculosis [J].
Collins, MT .
VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 1996, 12 (02) :357-+
[8]   Results of multiple diagnostic tests for Mycobacterium avium subsp paratuberculosis in patients with inflammatory bowel disease and in controls [J].
Collins, MT ;
Lisby, G ;
Moser, C ;
Chicks, D ;
Christensen, S ;
Reichelderfer, M ;
Hoiby, N ;
Harms, BA ;
Thomsen, OO ;
Skibsted, U ;
Binder, V .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (12) :4373-4381
[9]   DETECTION OF MYCOBACTERIUM-PARATUBERCULOSIS BY POLYMERASE CHAIN-REACTION IN CHILDREN WITH CROHNS-DISEASE [J].
DELLISOLA, B ;
POYART, C ;
GOULET, O ;
MOUGENOT, JF ;
SADOUNJOURNO, E ;
BROUSSE, N ;
SCHMITZ, J ;
RICOUR, C ;
BERCHE, P .
JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (02) :449-451
[10]   Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp paratuberculosis in bovine fecal samples [J].
Fang, Y ;
Wu, WH ;
Pepper, JL ;
Larsen, JL ;
Marras, SAE ;
Nelson, EA ;
Epperson, WB ;
Christopher-Hennings, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (01) :287-291