Immunisation against plague by transcutaneous and intradermal application of subunit antigens

被引:24
作者
Eyles, JE [1 ]
Elvin, SJ
Westwood, A
LeButt, CS
Alpar, HO
Somavarapu, S
Williamson, ED
机构
[1] Biomed Sci Dstl, Salisbury SP4 0JQ, Wilts, England
[2] Univ London, Sch Pharm, London WC1N 1AX, England
关键词
immunisation; non-invasive; skin; mucosal; enterotoxin; subunit vaccines;
D O I
10.1016/j.vaccine.2004.02.049
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have investigated immunological responses in BALB/c mice following transcutaneous (TC) delivery of fraction 1 (F1) and V subunits from Yersinia pestis in conjunction with an enterotoxin-derived adjuvant (cholera toxin, CT). It was found that two or more TC applications of F1 and V subunits (admixed with cholera toxin) served to elicit significant levels of anti-F1 and V antibodies in the serum of immunised mice. IL-6 secretion from cultured splenocytes derived from immunised mice indicated that a single TC application of F1 and V subunits (admixed with caolera toxin) conferred a cell-mediated response. As compared with intranasal or direct intradermal injection of F1 and V, the numbers of F1/V=specific antibody-forming cells in the spleens of animals immunised by TC application of F1 and V (admixed with CT) was relatively low. It was noted that TC application of F1 and V admixed with CT was very effective for priming responses that were boosted by intranasal or intradermal routes. Similarly, it was found that TC application of F1 and V admixed with CT could be used to efficiently boost pre-existing responses engendered by intradermal injection or intranasal instillation of F1 and V. In order to assess if TC application of F1 and V admixed with CT could protect experimental animals from plague, immunised mice were injected with a virulent strain of Y. pestis. It was found that two TC applications of F1 and V admixed with CT conferred only limited protection against 10(2) MLDs. However, three TC applications of F1 and V admixed with CT conferred solid protection against 102 MLDs. Hence we have shown, for the first time, that TC application of F: and V admixed with CT can protect animals against challenge with a virulent strain of plague causing bacteria. These data suggest that transcutaneous immunisation may be a simple and non-invasive method for immunising individuals against plague. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4365 / 4373
页数:9
相关论文
共 24 条
  • [1] Cutaneous vaccination: the skin as an immunologically active tissue and the challenge of antigen delivery
    Babiuk, S
    Baca-Estrada, M
    Babiuk, LA
    Ewen, C
    Foldvari, M
    [J]. JOURNAL OF CONTROLLED RELEASE, 2000, 66 (2-3) : 199 - 214
  • [2] Immunization onto bare skin with heat-labile enterotoxin of Escherichia coli enhances immune responses to coadministered protein and peptide antigens and protects mice against lethal toxin challenge
    Beignon, AS
    Briand, JP
    Muller, S
    Partidos, CD
    [J]. IMMUNOLOGY, 2001, 102 (03) : 344 - 351
  • [3] Epidermal immunization by a needle-free powder delivery technology: Immunogenicity of influenza vaccine and protection in mice
    Chen, DX
    Endres, RL
    Erickson, CA
    Weis, KF
    McGregor, MW
    Kawaoka, Y
    Payne, LG
    [J]. NATURE MEDICINE, 2000, 6 (10) : 1187 - 1190
  • [4] Manipulating systemic and mucosal immune responses with skin-deliverable adjuvants
    Chin, J
    SanGil, F
    Novak, M
    Eamens, G
    Djordjevic, S
    Simecka, J
    Duncan, J
    Mullbacher, A
    [J]. JOURNAL OF BIOTECHNOLOGY, 1996, 44 (1-3) : 13 - 19
  • [5] Analysis of local and systemic immunological responses after intra-tracheal, intra-nasal and intra-muscular administration of microsphere co-encapsulated Yersinia pestis sub-unit vaccines
    Eyles, JE
    Spiers, ID
    Williamson, ED
    Alpar, HO
    [J]. VACCINE, 1998, 16 (20) : 2000 - 2009
  • [6] Intra nasal administration of poly-lactic acid microsphere co-encapsulated Yersinia pestis subunits confers protection from pneumonic plague in the mouse
    Eyles, JE
    Sharp, GJE
    Williamson, ED
    Spiers, ID
    Alpar, HO
    [J]. VACCINE, 1998, 16 (07) : 698 - 707
  • [7] Protection studies following bronchopulmonary and intramuscular immunisation with Yersinia pestis F1 and V subunit vaccines coencapsulated in biodegradable microspheres:: a comparison of efficacy
    Eyles, JE
    Williamson, ED
    Spiers, ID
    Alpar, HO
    [J]. VACCINE, 2000, 18 (28) : 3266 - 3271
  • [8] Microsphere translocation and immunopotentiation in systemic tissues following intranasal administration
    Eyles, JE
    Bramwell, VW
    Williamson, ED
    Alpar, HO
    [J]. VACCINE, 2001, 19 (32) : 4732 - 4742
  • [9] Transcutaneous immunization: A human vaccine delivery strategy using a patch
    Glenn, GM
    Taylor, DN
    Li, XR
    Frankel, S
    Montemarano, A
    Alving, CR
    [J]. NATURE MEDICINE, 2000, 6 (12) : 1403 - 1406
  • [10] Transcutaneous immunization: T cell responses and boosting of existing immunity
    Hammond, SA
    Walwender, D
    Alving, CR
    Glenn, GM
    [J]. VACCINE, 2001, 19 (17-19) : 2701 - 2707