Ritonavir does not inhibit calpain in vitro

被引：8

作者：

Cuerrier, D

Nie, Z

Badley, AD

Davies, PL ^{[1
]}

机构：

[1] Queens Univ, Dept Biochem, Kingston, ON K7L 3N6, Canada

[2] Mayo Clin & Mayo Fdn, Div Infect Dis, Rochester, MN 55905 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 327卷 / 01期

关键词：

calpain; protease; ritonavir; inhibitor; autolysis;

D O I：

10.1016/j.bbrc.2004.11.161

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ritonavir, an inhibitor of HIV-1 protease, has been reported to also inhibit the Ca2+-dependent cysteine protease, calpain. We have investigated these claims with an in vitro study of the effect of ritonavir on the m-calpain and mu-calpain isoforms. Ritonavir failed to block either autolytic or hydrolytic calpain activity, but remained fully capable of inhibiting the HIV-1 protease. Any calpain-related effects of ritonavir in cells must, therefore, arise by a mechanism other than direct inhibition of calpains. (C) 2004 Elsevier Inc. All rights reserved.

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收藏

页码：208 / 211

页数：4

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