Effect of sample preparation, length of time, and sample size on quantification of total lipids from bovine liver

被引：35

作者：

Ametaj, BN ^{[1
]}

Bobe, G ^{[1
]}

Lu, Y ^{[1
]}

Young, JW ^{[1
]}

Beitz, DC ^{[1
]}

机构：

[1] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2003年 / 51卷 / 08期

关键词：

bovine; extraction; homogenization; liver; pulverization; quantification; sonication; total lipids;

D O I：

10.1021/jf0259011

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The objective was to evaluate the effect of sample preparation (pulverization under liquid nitrogen, homogenization, or sonication), time length of sonication (0-60 s), shaking in chloroform/methanol solvent (0, 2, 4, or 12 h), incubation in chloroform (0 or 12 h), and drying of extracted lipids at 50 degreesC (2, 4, 6, or 24 h), and sample size (50-250 mg) on quantification of total lipids from bovine liver. Pulverization under liquid nitrogen yielded the lowest recovery. Sonication was least time-consuming for sample preparation. Precise estimates and the greatest recovery were obtained with 30 s of sonication, at least 2 h of shaking in chloroform/methanol solvent, 12 h of incubation in chloroform, and at least 6 h of drying. Sample sizes of at least 150 mg gave precise estimates. The results demonstrate that sample preparation, time length of different steps of the extraction procedure, and sample size affect quantification of total lipid from bovine liver.

引用

页码：2105 / 2110

页数：6

共 33 条

[1] RAPID METHOD FOR ACCURATE DETERMINATION OF LIPID IN ANIMAL TISSUES [J].