Second-site eversion of a structural defect in bacteriophage T4 lysozyme

被引：5

作者：

Bouvier, SE ^{[1
]}

Poteete, AR ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS,MED CTR,DEPT MOLEC GENET & MICROBIOL,WORCESTER,MA 01655

来源：

FASEB JOURNAL | 1996年 / 10卷 / 01期

关键词：

lysozyme; vector; mutation;

D O I：

10.1096/fasebj.10.1.8566537

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A plasmid-borne gene encoding bacteriophage T4 lysozyme with a structural mutation, Tyr(161)-Ala, was mutagenized by the use of polymerase chain reaction, The mutagenized gene was inserted into a specialized bacteriophage lambda cloning vector that must acquire a functional lysozyme gene in order to form plaques, Functional variants of the mutant lysozyme were selected. Three compensatory second-site revertants were obtained: Thr(152)-Met, Lys(43)-Ile, and Thr(151)-Ala. The effects of these mutations are interpreted in light of previous structural and genetic studies of T4 lysozyme.

引用

页码：159 / 163

页数：5

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