Myc-induced T cell leukemia in transgenic zebrafish

被引：412

作者：

Langenau, DM

Traver, D

Ferrando, AA

Kutok, JL

Aster, JC

Kanki, JP

Lin, S

Prochownik, E

Trede, NS

Zon, LI

Look, AT

机构：

[1] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA

[2] Childrens Hosp Pittsburgh, Div Hematol Oncol, Pittsburgh, PA 15213 USA

[3] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[4] Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA

[5] Harvard Univ, Sch Med, Childrens Hosp Boston, Div Hematol Oncol, Boston, MA 02115 USA

来源：

SCIENCE | 2003年 / 299卷 / 5608期

关键词：

D O I：

10.1126/science.1080280

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The zebrafish is an attractive model organism for studying cancer development because of its genetic accessibility. Here we describe the induction of clonally derived T cell acute lymphoblastic leukemia in transgenic zebrafish expressing mouse c-myc under control of the zebrafish Rag2 promoter. Visualization of leukemic cells expressing a chimeric transgene encoding Myc fused to green fluorescent protein (GFP) revealed that leukemias arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into skeletal muscle and abdominal organs. Leukemic cells homed back to the thymus in irradiated fish transplanted with GFP-labeled leukemic lymphoblasts. This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-myc-induced carcinogenesis.

引用

页码：887 / 890

页数：4

共 24 条

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