Enzymatic resolution of Z-gamma,gamma'-di-tert-butyl-D,L-carboxyglutamic acid methyl ester

被引:10
作者
Clapes, P [1 ]
Valverde, I [1 ]
Jaime, C [1 ]
Torres, JL [1 ]
机构
[1] UNIV AUTONOMA BARCELONA, FAC SCI, DEPT CHEM, E-08193 BARCELONA, SPAIN
关键词
D O I
10.1016/0040-4039(95)02175-2
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
A rapid and simple method for the resolution of Z-gamma,gamma'-di-tert-butyl-D,L-carboxyglutamic acid methyl ester is described. The new procedure is based on the enzymatic enantioselective saponification of the methyl ester by the endoprotease papain. Using a simple HPLC protocol the enantiomeric excess or Z-di-tert-butyl-L-Gla-OH has been shown to be higher than 99.5%. The procedures described in this communication allow the resolution, analysis, and downstream process of multi-gram amounts of the Gla derivative in 48 h.
引用
收藏
页码:417 / 418
页数:2
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