Analysis of protein profiles of genetically modified potato tubers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引:16
作者
Careri, M
Elviri, L
Mangia, A
Zagnoni, I
Agrimonti, C
Visioli, G
Marmiroli, N
机构
[1] Univ Parma, Dipartimento Chim Gen & Inorgan, I-43100 Parma, Italy
[2] Univ Parma, Dipartimento Sci Ambientali, Sez Genet & Biotecnol Ambientali, I-43100 Parma, Italy
关键词
D O I
10.1002/rcm.944
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Traceability of genetically modified (GM) foods demands the development of appropriate reliable techniques in order to identify and quantify peptide or nucleic acid residues in GM plants and food products through the food chain. In this study the applicability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was demonstrated for the characterization of proteins of transformed and untransformed potato (Solanum Tuberosum L.) tubers. In GM tubers the expression level of the G1-1 gene, which regulates transition from dormancy to sprouting tubers, was inhibited by antisense technology. The analysis of antisense transformed lines showed that several of them exhibited a significant delay in sprouting relative to the control lines, in accordance with a decrease in the transcript level. Preliminary attempts to compare the protein patterns obtained from transformed and control lines using traditional electrophoresis were not able to reveal differences in the low-kDa range. Instead, MALDI-TOFMS applied to total peptide extract without any purification was able to distinguish spectral patterns of transformed and untransformed lines. In particular, several characteristic peaks from m/z 4373 to 4932 were detected only in the mass spectra of GM tuber samples. Copyright (C) 2003 John Wiley Sons, Ltd.
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收藏
页码:479 / 483
页数:5
相关论文
共 11 条
  • [1] In vitro and in silico analysis of two genes (A2-1 and G1-1) differentially regulated during dormancy and sprouting in potato tubers
    Agrimonti, C
    Visioli, G
    Marmiroli, N
    [J]. POTATO RESEARCH, 2000, 43 (04) : 325 - 333
  • [2] Functional genomics by mass spectrometry
    Andersen, JS
    Mann, M
    [J]. FEBS LETTERS, 2000, 480 (01) : 25 - 31
  • [3] The Molecular Biology Database Collection: an updated compilation of biological database resources
    Baxevanis, AD
    [J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (01) : 1 - 10
  • [4] Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low-oxygen environment, and identification of proteins by mass spectrometry
    Chang, WWP
    Huang, L
    Shen, M
    Webster, C
    Burlingame, AL
    Roberts, JKM
    [J]. PLANT PHYSIOLOGY, 2000, 122 (02) : 295 - 317
  • [5] Gene2EST:: a BLAST2 server for searching expressed sequence tag (EST) databases with eukaryotic gene-sized queries
    Gemünd, C
    Ramu, C
    Altenberg-Greulich, B
    Gibson, TJ
    [J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (06) : 1272 - 1277
  • [6] Advances in proteome analysis by mass spectrometry
    Griffin, TJ
    Aebersold, R
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (49) : 45497 - 45500
  • [7] Correlation between protein and mRNA abundance in yeast
    Gygi, SP
    Rochon, Y
    Franza, BR
    Aebersold, R
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1999, 19 (03) : 1720 - 1730
  • [8] INHIBITION OF THYMIDINE KINASE GENE-EXPRESSION BY ANTI-SENSE RNA - A MOLECULAR APPROACH TO GENETIC-ANALYSIS
    IZANT, JG
    WEINTRAUB, H
    [J]. CELL, 1984, 36 (04) : 1007 - 1015
  • [9] Silencing of G1-1 and A2-1 genes.: Effects on general plant phenotype and on tuber dormancy in Solanum tuberosum L.
    Marmiroli, N
    Agrimonti, C
    Visioli, G
    Colauzzi, M
    Guarda, G
    Zuppini, A
    [J]. POTATO RESEARCH, 2000, 43 (04) : 313 - 323
  • [10] GENES GALORE - A SUMMARY OF METHODS FOR ACCESSING RESULTS FROM LARGE-SCALE PARTIAL SEQUENCING OF ANONYMOUS ARABIDOPSIS CDNA CLONES
    NEWMAN, T
    DEBRUIJN, FJ
    GREEN, P
    KEEGSTRA, K
    KENDE, H
    MCINTOSH, L
    OHLROGGE, J
    RAIKHEL, N
    SOMERVILLE, S
    THOMASHOW, M
    RETZEL, E
    SOMERVILLE, C
    [J]. PLANT PHYSIOLOGY, 1994, 106 (04) : 1241 - 1255