In vitro toxicity of respirable-size particles of diatomaceous earth and crystalline silica compared with asbestos and titanium dioxide

The relationship between particle characteristics and in vitro toxicity was investigated using Chinese hamster ovary cells. Test dusts included respirable natural (Nat) and flux-calcined (FC) diatomaceous earth (DE), quartz, cristobalite, TiO(2), and chrysotile and crocidolite asbestos. All dusts elicited a qualitatively similar, concentration-dependent response: particle uptake, induction of micro-and polynuclei, and reduction in cell proliferation. However, similar mass concentrations of the dusts yielded a 35-fold range of toxicity: chrysotile>crocidolite>Nat DE>FC DE>quartz>Cristobalite>TiO(2). In vitro toxicity did not correlate with crystalline silica content, surface area, composition, volume, particles/cm(2), or fibrous geometry. Toxicity was closely associated with the number of particles/cm(2) culture surface that had at least one dimension>7.5 mu m. Thus particle size but not shape could be a determinant of in vitro toxicity. Particle size might also impact in vivo pathogenesis.