Characteristics of arylsulfatase in Russian sturgeon (Acipenser gueldenstaedti) semen

被引：16

作者：

Sarosiek, B

Ciereszko, A

Kolman, R

Glogowski, J

机构：

[1] Polish Acad Sci, Dept Mol Androl, Inst Anim Reprod & Food Res, PL-10243 Olsztyn, Poland

[2] Polish Acad Sci, Semen Biol Grp, Inst Anim Reprod & Food Res, PL-10243 Olsztyn, Poland

[3] Inland Fisheries Inst, Olsztyn, Poland

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2004年 / 139卷 / 04期

关键词：

arylsulfatase; milt plasma; Russian sturgeon; spermatozoa; acrosome; semen; acipenseridae; milt;

D O I：

10.1016/j.cbpc.2004.03.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spermatozoa of sturgeons (Acipenscriformes), unlike teleosts, possess an acrosome. This paper provides data concerning biochemical characteristics of arylsulfatase (AS), an acrosomal enzyme, found in Russian sturgeon spermatozoa and seminal plasma. The enzymes were purified by a four-step procedure, using n-butanol extraction, ion-exchange chromatography repeated twice and gel filtration. High purity of our enzymes was confirmed by silver staining electrophoresis and an immunological experiment. Kinetic parameters indicated that the purified enzymes belong to arylsulfatase type A. Similarity of the seminal plasma arylsulfatase to the spermatozoan enzyme showed us that arylsulfatase from seminal plasma might originate from damaged spermatozoa. The possible physiological consequences of the presence of arylsulfatase in Russian sturgeon semen are discussed. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：571 / 579

页数：9

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