Interaction of peroxynitrite with carotenoids and tocopherols within low density lipoprotein

被引:48
作者
Pannala, AS
Rice-Evans, C
Sampson, J
Singh, S
机构
[1] Univ London Kings Coll, Dept Pharm, London SW3 6LX, England
[2] Guys Hosp, UMDS, Int Antioxidant Res Ctr, London SE1 9RT, England
关键词
carotenoid; low density lipoprotein oxidation; peroxynitrite; tocopherol; lipid peroxidation;
D O I
10.1016/S0014-5793(98)00108-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Peroxynitrite is a powerful oxidising and nitrating agent generated in vivo by the combination of nitric oxide and superoxide, Previous studies have shown that on exposure to peroxynitrite, low density lipoprotein (LDL) is modified resulting in both a time- and concentration-dependent change to lipid and protein components, The present investigation highlights the reaction between carotenoids and tocopherols, present within the lipophilic phase of LDL, and peroxynitrite at varying concentrations, It was observed that the carotenoids were consumed by a significantly greater proportion than that of the tocopherols with lycopene (87.2+/-11%) being more reactive than beta-carotene (68.2+/-5.8%) when exposed to peroxynitrite (50 mu M) for 1 min. Among the tocopherols, alpha-tocopherol (54.9+/-20.2%) was more extensively depleted than gamma-tocopherol (14.7+/-1.09%) at peroxynitrite concentration of 500 mu M., It was also observed that peroxynitrite, unlike copper ions, does not lead to significant peroxidation of LDL as determined by the formation of conjugated dienes and thiobarbituric acid-reactive substances. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:297 / 301
页数:5
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