Single-molecule mass spectrometry in solution using a solitary nanopore

被引：304

作者：

Robertson, Joseph W. F.

Rodrigues, Claudio G.

Stanford, Vincent M.

Rubinson, Kenneth A.

Krasilnikov, Oleg V. ^{[1
]}

Kasianowicz, John J.

机构：

[1] Natl Inst Standards & Technol, Semicond Elect Div, Elect & Elect Engn Lab, Gaithersburg, MD 20899 USA

[2] Univ Fed Pernambuco, Dept Biophys & Radiobiol, Lab Membrane Biophys, BR-50670901 Recife, PE, Brazil

[3] Natl Inst Standards & Technol, Informat Access Div, Informat Technol Lab, Gaithersburg, MD 20899 USA

[4] Wright State Univ, Dept Biochem & Mol Biol, Dayton, OH 45435 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 20期

关键词：

Gaussian mixture model; poly(ethylene glycol); alpha-hemolysin; ion channel; maximum likelihood signal classification;

D O I：

10.1073/pnas.0611085104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single a-hemolysin pore cause distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat unit of ethylene glycol, and the mean residence time increases monotonically with the poly(ethylene glycol) mass. This technique could prove useful for the real-time characterization of molecules in solution.

引用

页码：8207 / 8211

页数：5

共 32 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].

Akeson, M ;

Branton, D ;

Kasianowicz, JJ ;

Brandin, E ;

Deamer, DW .

BIOPHYSICAL JOURNAL, 1999, 77 (06) :3227-3233

[2]

Baum L.E., 1972, Inequalities III: Proceedings of the Third Symposium on Inequalities, page, V3, P1

[3] COUNTING POLYMERS MOVING THROUGH A SINGLE-ION CHANNEL [J].

BEZRUKOV, SM ;

VODYANOY, I ;

PARSEGIAN, VA .

NATURE, 1994, 370 (6487) :279-281

[4] Dynamics and free energy of polymers partitioning into a nanoscale pore [J].

Bezrukov, SM ;

Vodyanoy, I ;

Brutyan, RA ;

Kasianowicz, JJ .

MACROMOLECULES, 1996, 29 (26) :8517-8522

[5]

Bezrukov SM, 2002, NATO SCI S PRT 3 HI, V87, P117

[6]

CHAKARAVARTI IM, 1967, HDB METHODS APPL STA

[7]

Coulter WH., 1953, Means for counting particles suspended in a fluid, Patent No. [2656508, 2656508A]

[8]

Einstein Albert., 1956, INVESTIGATION THEORY

[9] Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter [J].

Gu, LQ ;

Braha, O ;

Conlan, S ;

Cheley, S ;

Bayley, H .

NATURE, 1999, 398 (6729) :686-690

[10] Anthrax biosensor, protective antigen ion channel asymmetric blockade [J].

Halverson, KM ;

Panchal, RG ;

Nguyen, TL ;

Gussio, R ;

Little, SF ;

Misakian, M ;

Bavari, S ;

Kasianowicz, JJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (40) :34056-34062

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