Voltammetric determination of phytochelatins using copper solid amalgam electrode

被引：52

作者：

Yosypchuk, B ^{[1
]}

Sestáková, I ^{[1
]}

Novotny, L ^{[1
]}

机构：

[1] Acad Sci Czech Republ, J Heyrovsky Inst Phys Chem, CR-18223 Prague 8, Czech Republic

来源：

TALANTA | 2003年 / 59卷 / 06期

关键词：

phytochelatins; cathodic stripping; copper solid amalgam electrode;

D O I：

10.1016/S0039-9140(03)00023-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Voltammetric determination of synthetically prepared phytochelatins (gamma-Glu-Cys)(2)Gly (PC2) and (gamma-Glu-Cys)(3)Gly (PC3) has been studied using new type of copper solid amalgam electrode. The determination, based on the formation of cuprous complexes in buffer pH 8.1, is suitable for concentrations of PC in the range 10-100 nmol l(-1). Reproducibility, employing electrochemical cleaning of the electrode surface, was statistically evaluated. The achieved limit of detection (2.1-2.6 x 10(-9) mol l(-1) for DCV measurement) together with the robust character of the electrode offer its use for detection of PCs in separated extracts of real samples. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：1253 / 1258

页数：6

共 19 条

[1] PHYTOCHELATIN PRODUCTION BY MARINE-PHYTOPLANKTON AT LOW FREE METAL-ION CONCENTRATIONS - LABORATORY STUDIES AND FIELD DATA FROM MASSACHUSETTS BAY [J].

AHNER, BA ;

PRICE, NM ;

MOREL, FMM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (18) :8433-8436

[2] Phytochelatins and their roles in heavy metal detoxification [J].

Cobbett, CS .

PLANT PHYSIOLOGY, 2000, 123 (03) :825-832

[3] Differential pulse voltammetric study of the complexation of Cd(II) by the phytochelatin (γ-Glu-Cys)2Gly assisted by multivariate curve resolution [J].

Cruz, BH ;

Diaz-Cruz, JM ;

Sestáková, I ;

Velek, J ;

Ariño, C ;

Esteban, M .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2002, 520 (1-2) :111-118

[4] THE STRIPPING VOLTAMMETRIC PATTERN OF CYSTEINE AND PENICILLAMINE AT THE MERCURY-ELECTRODE IN THE PRESENCE OF CU(II) [J].

FORSMAN, U .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1983, 152 (1-2) :241-254

[5] PHYTOCHELATINS - THE PRINCIPAL HEAVY-METAL COMPLEXING PEPTIDES OF HIGHER-PLANTS [J].

GRILL, E ;

WINNACKER, EL ;

ZENK, MH .

SCIENCE, 1985, 230 (4726) :674-676

[6] PHYTOCHELATINS, A CLASS OF HEAVY-METAL-BINDING PEPTIDES FROM PLANTS, ARE FUNCTIONALLY ANALOGOUS TO METALLOTHIONEINS [J].

GRILL, E ;

WINNACKER, EL ;

ZENK, MH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (02) :439-443

[7]

Jocelyn P.C., 1972, Biochemistry of the SH group: the occurrence, chemical properties, metabolism and biological function of thiols and disulphides

[8] CATHODIC STRIPPING VOLTAMMETRY OF GLUTATHIONE IN NATURAL-WATERS [J].

LEGALL, AC ;

VAN DEN BERG, CMG .

ANALYST, 1993, 118 (11) :1411-1415

[9]

Merrifield Bruce, 1995, P93, DOI 10.1016/B978-012310920-0/50004-8

[10] PHYTOCHELATINS [J].

RAUSER, WE .

ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :61-86

← 1 2 →