The application of succession analysis in combination with EHF irradiation to the selective isolation of actinomycetes from soil

A new method employing succession analysis and extremely high frequency (EHF) irradiation is proposed for the selective isolation of actinomycetes from soil. Total actinomycetes were efficiently isolated from soil suspensions irradiated in the wavelength band 4.6-5.8 mm on the 14th and 45th days of succession initiated by soil wetting and from soil suspensions irradiated in the wavelength band 8-11.5 mm on the 7th day of succession. The rare actinomycete genera Actinomadura, Micromonospora, Nonomuraea, Micrabispora, Amycolatopsis, Pseudonocardia Saccharothrix, Streptosporangium, Actinosynnema, Nocardiordes, and Saccharopolyspora were isolated by either of the two approaches (succession analysis and EHF irradiation); however, the, range of isolated rare actinomycetes was considerably wider when a combination of the two approaches was used. For instance, actinomycetes of the rare genera Actinocorallia, Promicromonospora Actinoplanes, and Kibdelosporangium were isolated only when EHF irradiation was employed at the early stages of succession.