Macrodomain organization of the Escherichia coli chromosome

被引:257
作者
Valens, M
Penaud, S
Rossignol, M
Cornet, F
Boccard, F
机构
[1] CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France
[2] CNRS, Lab Microbiol & Genet Mol, Toulouse, France
关键词
bacterial chromosome; chromatids cohesion; chromosome conformation; macrodomain; site-specific recombination;
D O I
10.1038/sj.emboj.7600434
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have explored the Escherichia coli chromosome architecture by genetic dissection, using a site-specific recombination system that reveals the spatial proximity of distant DNA sites and records interactions. By analysing the percentages of recombination between pairs of sites scattered over the chromosome, we observed that DNA interactions were restricted to within subregions of the chromosome. The results indicated an organization into a ring composed of four macrodomains and two less-structured regions. Two of the macrodomains defined by recombination efficiency are similar to the Ter and Ori macrodomains observed by FISH. Two newly characterized macrodomains flank the Ter macrodomain and two less-structured regions flank the Ori macrodomain. Also the interactions between sister chromatids are rare, suggesting that chromosome segregation quickly follows replication. These results reveal structural features that may be important for chromosome dynamics during the cell cycle.
引用
收藏
页码:4330 / 4341
页数:12
相关论文
共 33 条
[1]   ANALYSIS OF CELL-SIZE AND DNA CONTENT IN EXPONENTIALLY GROWING AND STATIONARY-PHASE BATCH CULTURES OF ESCHERICHIA-COLI [J].
AKERLUND, T ;
NORDSTROM, K ;
BERNANDER, R .
JOURNAL OF BACTERIOLOGY, 1995, 177 (23) :6791-6797
[2]  
Azaro M.A., 2002, MOBILE DNA-UK, P118, DOI DOI 10.1128/9781555817954.CH7
[3]   RacA, a bacterial protein that anchors chromosomes to the cell poles [J].
Ben-Yehuda, S ;
Rudner, DZ ;
Losick, R .
SCIENCE, 2003, 299 (5606) :532-536
[4]   Linear ordering and dynamic segregation of the bacterial chromosome [J].
Breier, AM ;
Cozzarelli, NR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (25) :9175-9176
[5]   Polarization of the Escherichia coli chromosome.: A view from the terminus [J].
Capiaux, H ;
Cornet, F ;
Corre, J ;
Guijo, MI ;
Pérals, K ;
Rebollo, JE ;
Louarn, JM .
BIOCHIMIE, 2001, 83 (02) :161-170
[6]   RETRACTED: The bacterial condensin MukBEF compacts DNA into a repetitive, stable structure (Retracted Article. See vol 307, pg 1409, 2005) [J].
Case, RB ;
Chang, YP ;
Smith, SB ;
Gore, J ;
Cozzarelli, NR ;
Bustamante, C .
SCIENCE, 2004, 305 (5681) :222-227
[7]   The topological mechanism of phage λ integrase [J].
Crisona, NJ ;
Weinberg, RL ;
Peter, BJ ;
Sumners, DW ;
Cozzarelli, NR .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 289 (04) :747-775
[8]   Tn7 transposition as a probe of cis interactions between widely separated (190 Kilobases apart) DNA sites in the Escherichia coli chromosome [J].
DeBoy, RT ;
Craig, NL .
JOURNAL OF BACTERIOLOGY, 1996, 178 (21) :6184-6191
[9]   Transcription-induced barriers to supercoil diffusion in the Salmonella typhimurium chromosome [J].
Deng, S ;
Stein, RA ;
Higgins, NP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (10) :3398-3403
[10]   Temporal regulation of topoisomerase IV activity in E-coli [J].
Espeli, O ;
Levine, C ;
Hassing, H ;
Marians, KJ .
MOLECULAR CELL, 2003, 11 (01) :189-201