Impact of oncogenes in tumor angiogenesis:: Mutant K-ras up-regulation of vascular endothelial growth factor vascular permeability factor is necessary, but not sufficient for tumorigenicity of human colorectal carcinoma cells

被引:209
作者
Okada, F
Rak, JW
St Croix, B
Lieubeau, B
Kaya, M
Roncari, L
Shirasawa, S
Sasazuki, T
Kerbel, RS
机构
[1] Sunnybrook Hlth Sci Ctr, Div Canc Biol Res, Toronto, ON M4N 3M5, Canada
[2] Kyushu Univ, Med Inst Bioregulat, Dept Genet, Fukuoka 812, Japan
关键词
D O I
10.1073/pnas.95.7.3609
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Targeted disruption of the single mutant K-ras allele in two human colorectal carcinoma cell lines (DLD-1 and HCT-116) leads to loss of tumorigenic competence in nude mice with retention of ability to grow indefinitely in monolayer culture, Because expression of the mutant K-ras oncogene in these cell lines is associated with marked up regulation of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF), we sought to determine whether this potent angiogenesis inducer plays a role in K-ras-dependent tumorigenic competence, Transfection of a VEGF(121) antisense expression vector into DLD-1 and HCT-116 cells resulted in suppression of VEGF/VPF production by a factor of 3- to 4-fold, The VEGF/VPF-deficient sublines, unlike the parental population or vector controls, were profoundly suppressed in their ability to form tumors in nude mice for as long as 6 months after cell injection, In contrast, in vitro growth of these sublines was unaffected, thus demonstrating the critical importance of VEGF/VPF as an angiogenic factor for HCT-116 and DLD-1 cells, Transfection of a full-length VEGF(121) cDNA into two nontumorigenic mutant K-ras knockout sublines resulted in a weak but detectable restoration of tumorigenic ability in vivo in a subset of the transfectants, with no consistent change in growth properties in vitro, The findings indicate that mutant ras-oncogene-dependent VEGF/VPF expression is necessary, but not sufficient for progressive tumor growth in vivo and highlight the relative contribution of oncogenes, such as mutant K-ras, to the process of tumor angiogenesis.
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页码:3609 / 3614
页数:6
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