Capsaicin hydrolysis by Candida antarctica lipase

被引:26
作者
Duarte, DR
Castillo, E
Bárzana, E
López-Munguía, A
机构
[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Bioingn, Cuernavaca 62271, Morelos, Mexico
[2] Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico
关键词
amide hydrolysis; Candida antarctica; capsaicin; lipase;
D O I
10.1023/A:1005622704504
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Capsaicin was hydrolysed by lipase B from Candida antarctica into vanillylamine and 8-methyl-6-trans-nonenoic acid. Conversions of 70% were obtained after 72 h at 70 degreesC in water but decreased to only 15% when capsaicin was solubilized in 15% (v/v) ethanol/water after 72 h at 45 degreesC. No activity occurred in chloroform/water mixtures. According to our knowledge, this is the first report concerning amide hydrolysis by a lipase.
引用
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页码:1811 / 1814
页数:4
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