Characterization and cytokine-stimulating activities of acidic heteroglycans from Tremella fuciformis

被引:45
作者
Gao, QP [1 ]
Killie, MK [1 ]
Chen, HC [1 ]
Jiang, RZ [1 ]
Seljelid, R [1 ]
机构
[1] UNIV TROMSO, INST MED BIOL, N-9037 TROMSO, NORWAY
关键词
Tremello fuciformis Berk; Tremellaceae; heteroglycans; interleukin-1 (IL-2); interleukin-6 (IL-6); tumor necrosis factor (TNF);
D O I
10.1055/s-2006-957733
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Four acidic heteroglycans, T2a-T2d, were isolated from the body of Tremella fuciformis Berk. They contained 1.9 %-2.9 % of acetyl groups and were composed of mannose (Man), glucuronic acid (GlcA), and small amounts of xylose (Xyl), glucose (Glc), and fucose (Fuc). According to methylation analysis they had a mannan backbone consisting of 3-linked Man, and side chains containing glucosyl, mannosyl, fucosyl, xylosyl, and glucuronic acid residues. The side chains were attached through 0-2, 0-4, or 0-6 in about 40 percent of backbone mannosyl residues. Molecular masses of the four polysaccharides were 410, 250, 34, and 20 kDa, respectively. T2a-T2d induced human monocytes to produce interleukin-l (IL-1), interleukin-6 (IL-6), and tumor necrosis factor (TNF) in vitro. The products of Smith degradation (T2a-S) and lithium degradation (T2a-L) of T2a and the product of deacetylation (T2b-D) of T2b also induced monocytes to secret IL-l as efficiently as the original polysaccharides, indicating that xylosyl and glucuronic acid residues as well as acetyl groups were not important to promote the cytokine-stimulating activity.
引用
收藏
页码:457 / 460
页数:4
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