Molecular and cellular basis of radiation fibrosis

Purpose: Recent data from the literature and the experimental work of the authors clearly indicate that TGF-beta 1 is a key modulator of cellular events, for example, induction of terminal differentiation, resulting in radiation-induced fibrosis. Therefore, the present study analysed which cellular processes induced by exogeneously added TGF-beta could be responsible for the induction, development and manifestation of the fibrotic phenotype in culture. Materials and methods: Rat lung fibroblast cultures (passage 1) were used. As a function of treatment with TGF-beta and/or anti-TGF-beta-antibody, the clonogenic activity and differentiation pattern were analysed by colony-formation assays. Results: It could be demonstrated that treatment of rat lung progenitor fibroblasts with TGF-beta 1 resulted in a pronounced shift in the differentiation pattern, i.e. induction of post-mitotic fibrocytes. This TGF-beta 1-dependent terminal differentiation could be abolished by simultaneous treatment with a neutralizing antibody directed against TGF-beta 1. Conclusions: The data presented indicate that TGF-beta 1 is one major candidate mediating the accelerated terminal differentiation of progenitor fibroblasts to post-mitotic functional fibrocytes, which results in the fibrotic phenotype of this cell system.