A method for determining intracellular concentrations of enzyme substrates from a combination of competitive inhibition and mutagenesis studies

被引：3

作者：

Copeland, RA

Luo, LS

Auger, KR

Huang, PS

机构：

[1] GlaxoSmithKline, Dept Enzymol & Mechanist Pharmacol, Collegeville, PA 19426 USA

[2] GlaxoSmithKline, Dept Oncol Biol, Collegeville, PA 19426 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 337卷 / 02期

关键词：

D O I：

10.1016/j.ab.2004.10.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

[No abstract available]

引用

页码：351 / 353

页数：3

共 8 条

[1]

CELLULAR CONCENTRATIONS OF ENZYMES AND THEIR SUBSTRATES [J].

ALBE, KR ;

BUTLER, MH ;

WRIGHT, BE .

JOURNAL OF THEORETICAL BIOLOGY, 1990, 143 (02) :163-195

[2]

Copeland R. A., 2004, ENZYMES PRACTICAL IN

[3]

CATALYSIS, BINDING AND ENZYME-SUBSTRATE COMPLEMENTARITY [J].

FERSHT, AR .

PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1974, 187 (1089) :397-407

[4]

Properties of cloned ATP-sensitive K+ currents expressed in Xenopus oocytes [J].

Gribble, FM ;

Ashfield, R ;

Ammala, C ;

Ashcroft, FM .

JOURNAL OF PHYSIOLOGY-LONDON, 1997, 498 (01) :87-98

[5]

Novel method for measurement of submembrane ATP concentration [J].

Gribble, FM ;

Loussouarn, G ;

Tucker, SJ ;

Zhao, C ;

Nichols, CG ;

Ashcroft, FM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (39) :30046-30049

[6]

Glucose generates sub-plasma membrane ATP microdomains in single islet β-cells -: Potential role for strategically located mitochondria [J].

Kennedy, HJ ;

Pouli, AE ;

Ainscow, EK ;

Jouaville, LS ;

Rizzuto, R ;

Rutter, GA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (19) :13281-13291

[7]

KUHNVELTEN WN, 1991, J BIOL CHEM, V266, P6291

[8]

IONIC CONTROL OF IMMOBILIZED ENZYMES - KINETICS OF ACID-PHOSPHATASE BOUND TO PLANT-CELL WALLS [J].

RICARD, J ;

NOAT, G ;

CRASNIER, M ;

JOB, D .

BIOCHEMICAL JOURNAL, 1981, 195 (02) :357-367

← 1 →