Improved determination of urinary cortisol and cortisone, or corticosterone and 11-dehydrocorticosterone by high-performance liquid chromatography with ultraviolet absorbance detection

被引:73
作者
Hay, M [1 ]
Mormède, P [1 ]
机构
[1] Inst Francois Magendie, INRA, INSERM, Lab Genet Stress, F-33077 Bordeaux, France
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 702卷 / 1-2期
关键词
cortisol; cortisone; corticosterone; 11-dehydrocorticosterone;
D O I
10.1016/S0378-4347(97)00361-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive assay was developed for the determination of low levels of free (unconjugated) glucocorticoids in human, swine (cortisol and cortisone) and rat urine (corticosterone and 11-dehydrocorticosterone), using solid-phase extraction and HPLC with UV absorbance detection (254 nm). Precise quantitation is allowed by the use of internal standards (dexamethasone for swine urine and Reichstein's substance S for rat urine). This simple method allows the use of small urine samples (less than 2 ml), and is suitable for a wide range of applications in human and animal clinical and physiological studies. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:33 / 39
页数:7
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