ATP-dependent potassium channel in rat cardiomyocytes is blocked by lidocaine - Possible impact on the antiarrhythmic action of lidocaine

Background During myocardial ischemia, lidocaine has favorable antiarrhythmic properties. Malignant arrhythmias result from heterogeneity between ischemic and nonischemic regions in extracellular potassium concentration and action potential duration. These effects have been attributed to the activation of ATP-dependent potassium (K-ATP) channels. In this study, we investigated the action of lidocaine on the K-ATP channels to test the possible link between the antiarrhythmic properties of lidocaine and its action on the K-ATP channel. Methods and Results The patch-clamp technique was em ployed on enzymatic dissociated cardiomyocytes of adult rats. Lidocaine was applied to the outer side of excised membrane patches by means of a multibarrel perfusion system. Lidocaine reversibly blocked the mean current of the K-ATP channels in a concentration-dependent manner (TC50=43+/-4.7 mu mol/L, E=0 mV, n=6), while the amplitude of the single-channel current remained unchanged. The half-maximum blocking concentration corresponds to the therapeutic range for the antiarrhythmic application of a lidocaine bolus in humans. Conclusions The open probability but not the conductance of the K-ATP channel in the membrane of rat cardiomyocytes is blocked by lidocaine. This action may explain, in part, the favor able antiarrhythmic properties of lidocaine during acute myocardial ischemia.