Spheroids in radiobiology and photodynamic therapy

被引:97
作者
Dubessy, C
Merlin, JL [1 ]
Marchal, C
Guillemin, F
机构
[1] Ctr Alexis Vautrin, Lab Rech Oncol, F-54511 Vandoeuvre Les Nancy, France
[2] Univ Nancy 1, Ctr Alexis Vautrin, UPRES B 2399, F-54511 Vandoeuvre Les Nancy, France
[3] Ctr Alexis Vautrin, Unite Rech Therapie Photodynam, F-54511 Vandoeuvre Les Nancy, France
[4] Dept Radiotherapie, F-54511 Vandoeuvre Les Nancy, France
[5] INPL, CNRS, UPRES A 7039, CRAN, F-54511 Vandoeuvre Les Nancy, France
关键词
oxygen; cell contact; apoptosis; damage repair;
D O I
10.1016/S1040-8428(00)00085-8
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Spheroids are tridimensional aggregates of tumor cells coming from one or several cell clones. This model, which mimics the micro-tumors structure and some of their properties, shows oxygen, pH and nutrient gradients inducing a necrotic area in the center of the spheroid. Analysis of spheroids, cultured under static or stirred conditions, can be performed on whole spheroids or dissociated spheroids. The spheroids sensitivity to ionizing radiation and photodynamic therapy can be altered by oxygen status, damage repair, intercellular communications and apoptosis induction, as in experimental tumor models. In radiobiology, the similarity of radiation response between spheroids and tumor xenograft bearing mice makes the spheroids to be a good alternative model to in vivo irradiation studies. In photodynamic therapy, spheroids lead to a better understanding of the own tumor response without interactions with vascular system. Finally, despite the quality of spheroid model, only the use of new technology for analysis of spheroid populations will help to increase their experimental use, particularly in preclinical oncology. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:179 / 192
页数:14
相关论文
共 134 条
[1]  
ACKER H, 1984, RECENT RESULTS CANC, V95, P116
[2]   COMPARATIVE PO2 MEASUREMENTS IN CELL SPHEROIDS CULTURED WITH DIFFERENT TECHNIQUES [J].
ACKER, H ;
CARLSSON, J ;
MUELLERKLIESER, W ;
SUTHERLAND, RM .
BRITISH JOURNAL OF CANCER, 1987, 56 (03) :325-327
[3]   APOPTOSIS INDUCED BY INHIBITION OF INTERCELLULAR CONTACT [J].
BATES, RC ;
BURET, A ;
VANHELDEN, DF ;
HORTON, MA ;
BURNS, GF .
JOURNAL OF CELL BIOLOGY, 1994, 125 (02) :403-415
[4]   A NEW METHOD FOR THE 3-D IN-VITRO GROWTH OF HUMAN RT112 BLADDER-CARCINOMA CELLS USING THE ALGINATE CULTURE TECHNIQUE [J].
BOXBERGER, HJ ;
MEYER, TF .
BIOLOGY OF THE CELL, 1994, 82 (2-3) :109-119
[5]  
BOXBERGER HJ, 1993, EUR J CELL BIOL, V62, P140
[6]   FORMATION AND GROWTH OF MULTICELLULAR SPHEROIDS OF HUMAN-ORIGIN [J].
CARLSSON, J ;
NILSSON, K ;
WESTERMARK, B ;
PONTEN, J ;
SUNDSTROM, C ;
LARSSON, E ;
BERGH, J ;
PAHLMAN, S ;
BUSCH, C ;
COLLINS, VP .
INTERNATIONAL JOURNAL OF CANCER, 1983, 31 (05) :523-533
[7]  
CARLSSON J, 1984, RECENT RESULTS CANC, V95, P1
[8]   Measuring hypoxia and predicting tumor radioresistance with nuclear medicine assays [J].
Chapman, JD ;
Engelhardt, EL ;
Stobbe, CC ;
Schneider, RF ;
Gerald, GE .
RADIOTHERAPY AND ONCOLOGY, 1998, 46 (03) :229-237
[9]  
COLE L, 1957, RADIAT RES, V3, P508
[10]  
Davies CD, 1997, ANTICANCER RES, V17, P4317