Structural insights into the assembly of the type III secretion needle complex

被引：295

作者：

Marlovits, TC

Kubori, T

Sukhan, A

Thomas, DR

Galán, JE

Unger, VM ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[2] Yale Univ, Sch Med, Sect Microbial Pathogenet, New Haven, CT 06536 USA

[3] Brandeis Univ, Rosenstiel Basic Med Sci Res Ctr, Waltham, MA 02454 USA

来源：

SCIENCE | 2004年 / 306卷 / 5698期

关键词：

D O I：

10.1126/science.1102610

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Type III secretion systems (TTSSs) mediate translocation of virulence factors into host cells. We report the 17-angstrom resolution structures of a central component of Salmonella typhimurium TTSS, the needle complex, and its assembly precursor, the bacterial envelope-anchored base. Both the base and the fully assembled needle complex adopted multiple oligomeric states in vivo, and needle assembly was accompanied by recruitment of the protein PrgJ as a structural component of the base. Moreover, conformational changes during needle assembly created scaffolds for anchoring both PrgJ and the needle substructure and may provide the basis for substrate-specificity switching during type III secretion.

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页码：1040 / 1042

页数：3

相关论文

共 14 条

[1] Structure and composition of the Shigella flexneri 'needle complex', a part of its type III secreton [J].

Blocker, A ;

Jouihri, N ;

Larquet, E ;

Gounon, P ;

Ebel, F ;

Parsot, C ;

Sansonetti, P ;

Allaoui, A .

MOLECULAR MICROBIOLOGY, 2001, 39 (03) :652-663

[2] Requirement for exported proteins in secretion through the invasion-associated type III system of Salmonella typhimurium [J].

Collazo, CM ;

Galan, JE .

INFECTION AND IMMUNITY, 1996, 64 (09) :3524-3531

[3] Assembly and function of type III secretory systems [J].

Cornelis, GR ;

Van Gijsegem, F .

ANNUAL REVIEW OF MICROBIOLOGY, 2000, 54 :735-774

[4] Type III secretion machines:: Bacterial devices for protein delivery into host cells [J].

Galán, JE ;

Collmer, A .

SCIENCE, 1999, 284 (5418) :1322-1328

[5] CLONING AND MOLECULAR CHARACTERIZATION OF GENES WHOSE PRODUCTS ALLOW SALMONELLA-TYPHIMURIUM TO PENETRATE TISSUE-CULTURE CELLS [J].

GALAN, JE ;

CURTISS, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6383-6387

[6] Salmonella interactions with host cells:: Type III secretion at work [J].

Galán, JE .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2001, 17 :53-86

[7] Contribution of Salmonella typhimurium type III secretion components to needle complex formation [J].

Kimbrough, TG ;

Miller, SI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (20) :11008-11013

[8] Supramolecular structure of the Salmonella typhimurium type III protein secretion system [J].

Kubori, T ;

Matsushima, Y ;

Nakamura, D ;

Uralil, J ;

Lara-Tejero, M ;

Sukhan, A ;

Galán, JE ;

Aizawa, S .

SCIENCE, 1998, 280 (5363) :602-605

[9] Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system [J].

Kubori, T ;

Sukhan, A ;

Aizawa, SI ;

Galán, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (18) :10225-10230

[10]

MARLOVITS TC, UNPUB