A role for tachykinins in the regulation of human sperm motility

被引:43
作者
Ravina, C. G.
Seda, M.
Pinto, F. M.
Orea, A.
Fernandez-Sanchez, M.
Pintado, C. O.
Candenas, M. Luz
机构
[1] Univ Seville, CSIC, Inst Invest Quim, Seville 41092, Spain
[2] Inst Valenciano Infertil, Seville 41011, Spain
[3] Univ Seville, Ctr Prod & Expt Animal, Seville 41807, Spain
关键词
human sperm; mRNA expression; tachykinins; tachykinin receptors;
D O I
10.1093/humrep/dem069
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
BACKGROUND: Tachykinins and tachykinin receptors are widely distributed in the male reproductive tract and appear to be involved in reproduction. However, the function and expression of tachykinins and their receptors in human spermatozoa remain poorly studied. We analysed the effects of tachykinins on sperm motility and characterized the population of tachykinin receptors in human spermatozoa. METHODS AND RESULTS: Motility analysis was performed following World Health Organization guidelines and we found that substance P (SP), human hemokinin-1 (hHK-1), neurokinin A (NKA) and neurokinin B (NKB) produced concentration-dependent increases in sperm progressive motility. The effects of tachykinins were antagonized by the NK1 receptor-selective antagonist SR 140333, the NK2 receptor-selective antagonist, SR 48968 and, to a lesser extent, also by the NK3 receptor-selective antagonist SR 142801. Immunocytochemistry studies showed expression of the NK1, NK2 and NK3 tachykinin receptor proteins in spermatozoa with different major sites of localization for each receptor. Western blot analysis confirmed the presence of tachykinin receptors in sperm cell homogenates. RT-PCR demonstrated expression of the genes that encode SP/NKA (TACI), NKB (TAC3) and hHK-1 (TAC4) but not the genes TACR1, TACR2 and TACR3 encoding NK1, NK2 and NK3 receptors, respectively. CONCLUSIONS: These results show for the first time that the NK1, NK2 and NK3 tachykinin receptor proteins are present in human spermatozoa. Our findings suggest that tachykinins, probably acting through these three tachykinin receptors, play a role in the regulation of human sperm motility.
引用
收藏
页码:1617 / 1625
页数:9
相关论文
共 40 条
[1]   KATP channels in mouse spermatogenic cells and sperm, and their role in capacitation [J].
Acevedo, JJ ;
Mendoza-Lujambio, I ;
de la Vega-Beltrán, JL ;
Treviño, CL ;
Felix, R ;
Darszon, A .
DEVELOPMENTAL BIOLOGY, 2006, 289 (02) :395-405
[2]   Expression and localization of δ-, κ-, and μ-opioid receptors in human spermatozoa and implications for sperm motility [J].
Agirregoitia, Ekaitz ;
Valdivia, Asier ;
Carracedo, Arkaitz ;
Casis, Luis ;
Gil, Javier ;
Subiran, Nerea ;
Ochoa, Carmen ;
Irazusta, Jon .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2006, 91 (12) :4969-4975
[3]   Expression and subcellular localization of the μ-opioid receptor in equine spermatozoa:: evidence for its functional role [J].
Albrizio, M ;
Guaricci, AC ;
Maritato, F ;
Sciorsci, RL ;
Mari, G ;
Calamita, N ;
Lacalandra, GM ;
Aiudi, GG ;
Minoia, R ;
Dell'Aquila, ME ;
Minoia, P .
REPRODUCTION, 2005, 129 (01) :39-49
[4]   Tachykinins and tachykinin receptors:: Structure and activity relationships [J].
Almeida, TA ;
Rojo, J ;
Nieto, PM ;
Pinto, FM ;
Hernandez, M ;
Martín, JD ;
Candenas, ML .
CURRENT MEDICINAL CHEMISTRY, 2004, 11 (15) :2045-2081
[5]  
[Anonymous], 1999, WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction
[6]   GONADAL-STEROID REGULATION OF SUBSTANCE-P (SP) AND SP-ENCODING MESSENGER RIBONUCLEIC-ACIDS IN THE RAT ANTERIOR-PITUITARY AND HYPOTHALAMUS [J].
BROWN, ER ;
HARLAN, RE ;
KRAUSE, JE .
ENDOCRINOLOGY, 1990, 126 (01) :330-340
[7]   Biological effects of recombinant human zona pellucida proteins on sperm function [J].
Caballero-Campo, P ;
Chirinos, M ;
Fan, XJ ;
González-González, ME ;
Galicia-Chavarría, M ;
Larrea, F ;
Gerton, GL .
BIOLOGY OF REPRODUCTION, 2006, 74 (04) :760-768
[8]   Tachykinins and tachykinin receptors: effects in the genitourinary tract [J].
Candenas, L ;
Lecci, A ;
Pinto, FM ;
Patak, E ;
Maggi, CA ;
Pennefather, JN .
LIFE SCIENCES, 2005, 76 (08) :835-862
[9]   TACHYKININ (SUBSTANCE-P) GENE-EXPRESSION IN LEYDIG-CELLS OF THE HUMAN AND MOUSE TESTIS [J].
CHIWAKATA, C ;
BRACKMANN, B ;
HUNT, N ;
DAVIDOFF, M ;
SCHULZE, W ;
IVELL, R .
ENDOCRINOLOGY, 1991, 128 (05) :2441-2448
[10]   Calcium channels and Ca2+ fluctuations in sperm physiology [J].
Darszon, A ;
Nishigaki, T ;
Wood, C ;
Treviño, CL ;
Felix, R ;
Beltrán, C .
INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 243, 2005, 243 :79-172