MALDI-TOF MS of Trichoderma: a model system for the identification of microfungi

被引:50
作者
De Respinis, Sophie [1 ]
Vogel, Guido [2 ]
Benagli, Cinzia [1 ]
Tonolla, Mauro [1 ,3 ]
Petrini, Orlando [1 ]
Samuels, Gary J. [4 ]
机构
[1] Cantonal Inst Microbiol, CH-6500 Bellinzona, Switzerland
[2] Mabritec AG, CH-4002 Basel, Switzerland
[3] Univ Geneva, Dept Plant Biol, Microbial Ecol Lab, Microbiol Unit, CH-1211 Geneva 4, Switzerland
[4] USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA
关键词
Taxonomy; MALDI-TOFMS; Mass spectrometry; Hypocreales; TIME-OF-FLIGHT; POLYPEPTIDE ANTIBIOTICS PEPTAIBIOTICS; MASS-SPECTROMETRY; GENUS TRICHODERMA; FUTURE-PROSPECTS; HYPOCREA; LONGIBRACHIATUM; SPORES; DIVERSITY; PHYLOGENY;
D O I
10.1007/s11557-009-0621-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This investigation aimed to assess whether MALDI-TOF MS analysis of the proteome could be applied to the study of Trichoderma, a fungal genus selected because it includes many species and is phylogenetically well defined. We also investigated whether MALDI-TOF MS analysis of peptide mass fingerprints would reveal apomorphies that could be useful in diagnosing species in this genus. One hundred and twenty nine morphologically and genetically well-characterized strains of Hypocrea and Trichoderma, belonging to 25 species in 8 phylogenetic clades, were analyzed by MALDI-TOF MS mass spectrometry. The resulting peak lists of individual samples were submitted to single-linkage cluster analysis to produce a taxonomic tree and were compared to ITS and tef1 sequences from GenBank. SuperSpectra (TM) for the 13 most relevant species of Trichoderma were computed. The results confirmed roughly previously defined clades and sections. With the exceptions of T. saturnisporum (Longibrachiatum Clade) and T. harzianum ( Harzianum Clade), strains of individual species clustered very closely. T. polysporum clustered distantly from all other groups. The MALDI-TOF MS analysis accurately reflected the phylogenetic classification reported in recent publications, and, in most cases, strains identified by DNA sequence analysis clustered together by MALDI-TOF MS. The resolution of MALDI-TOF MS, as performed here, was roughly equivalent to ITS rDNA. The MALDI-TOF MS technique analyzes peptides and represents a rough equivalent to sequencing, making this method a useful adjunct for determination of species limits. It also allows simple, reliable, and quick species identification, thus representing a valid alternative to gene sequencing for species diagnosis of Trichoderma and other fungal taxa.
引用
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页码:79 / 100
页数:22
相关论文
共 67 条
  • [1] Benitez T., 1998, Trichoderma and gliocladium: enzymes, biological control and commercial applications, V2, P101
  • [2] BIGLY P, 1998, TRICHODERMA GLIOCLAD, P25
  • [3] A REVISION OF THE GENUS TRICHODERMA .3. SECTION PACHYBASIUM
    BISSETT, J
    [J]. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1991, 69 (11): : 2373 - 2417
  • [4] Rapid typing of bacteria using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry and pattern recognition software
    Bright, JJ
    Claydon, MA
    Soufian, M
    Gordon, DB
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2002, 48 (2-3) : 127 - 138
  • [5] Occupational Sensitization to fungal enzymes used in animal feed industry
    Caballero, Maria Luisa
    Gomez, Maria
    Gonzalez-Munoz, Miguel
    Reinoso, Luis
    Rodriguez-Perez, Rosa
    Alday, Enrique
    Moneo, Ignacio
    [J]. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2007, 144 (03) : 231 - 239
  • [6] Multilocus phylogenetic structure within the Trichoderma harzianum/Hypocrea lixii complex
    Chaverri, P
    Castlebury, LA
    Samuels, GJ
    Geiser, DM
    [J]. MOLECULAR PHYLOGENETICS AND EVOLUTION, 2003, 27 (02) : 302 - 313
  • [7] CHAVERRI P, 2003, STUD MYCOL, V48, P1
  • [8] Characterization of intact Penicillium spores by matrix-assisted laser desorption/ionization mass spectrometry
    Chen, HY
    Chen, YC
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (23) : 3564 - 3568
  • [9] Unraveling evolutionary relationships among the divergent lineages of Colletotrichum causing anthracnose disease in turfgrass and corn
    Crouch, JA
    Clarke, BB
    Hillman, BI
    [J]. PHYTOPATHOLOGY, 2006, 96 (01) : 46 - 60
  • [10] Genetic and biological diversity of Trichoderma stromaticum, a mycoparasite of the cacao witches'-broom pathogen
    de Souza, JT
    Pomella, AWV
    Bowers, JH
    Pirovani, CP
    Loguercio, LL
    Hebbar, KP
    [J]. PHYTOPATHOLOGY, 2006, 96 (01) : 61 - 67