Artificial evolution by DNA shuffling

被引：66

作者：

Harayama, S ^{[1
]}

机构：

[1] Marine Biotechnol Inst, Kamaishi, Iwate 026, Japan

来源：

TRENDS IN BIOTECHNOLOGY | 1998年 / 16卷 / 02期

关键词：

D O I：

10.1016/S0167-7799(97)01158-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Improvement of enzymes is one of the important objectives of biotechnology. In vitro evolution of enzymes using DNA shuffling involves the assembly of two or more DNA segments into a full-length gene by homologous, or site-specific, recombination, Before the assembly, the segments are often subjected to random mutagenesis by error-prone PCR, random nucleotide insertion or other methods, Many useful enzymes and peptides have been isolated following the artificial evolution.

引用

页码：76 / 82

页数：7

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