A Single-Cell Transcriptome Atlas of the Aging Drosophila Brain

被引：485

作者：

Davie, Kristofer ^{[1
,2
]}

Janssens, Jasper ^{[1
,2
]}

Koldere, Duygu ^{[1
,2
]}

De Waegeneer, Maxime ^{[1
,2
]}

Pech, Uli ^{[1
,3
]}

Kreft, Lukasz ^{[4
]}

Aibar, Sara ^{[1
,2
]}

Makhzami, Samira ^{[1
,2
]}

Christiaens, Valerie ^{[1
,2
]}

Gonzalez-Blas, Carmen Bravo ^{[1
,2
]}

Poovathingal, Suresh ^{[1
]}

Hulselmans, Gert ^{[1
,2
]}

Spanier, Katina I. ^{[1
,2
]}

Moerman, Thomas ^{[5
,6
]}

Vanspauwen, Bram ^{[7
]}

Geurs, Sarah ^{[2
]}

Voet, Thierry ^{[2
]}

Lammertyn, Jeroen ^{[7
]}

Thienpont, Bernard ^{[2
]}

Liu, Sha ^{[1
,3
]}

Konstantinides, Nikos ^{[8
]}

Fiers, Mark ^{[1
,3
]}

Verstreken, Patrik ^{[1
,3
]}

Aerts, Stein ^{[1
,2
]}

机构：

[1] Katholieke Univ Leuven, VIB Ctr Brain & Dis Res, B-3000 Leuven, Belgium

[2] Katholieke Univ Leuven, Dept Human Genet, B-3000 Leuven, Belgium

[3] Katholieke Univ Leuven, Dept Neurosci, B-3000 Leuven, Belgium

[4] VIB, VIB Bioinformat Core, B-9052 Ghent, Belgium

[5] Katholieke Univ Leuven, ESAT, B-3001 Leuven, Belgium

[6] IMEC, Smart Applicat & Innovat Serv, B-3001 Leuven, Belgium

[7] Katholieke Univ Leuven, Dept Biosyst, B-3001 Leuven, Belgium

[8] NYU, Dept Biol, New York, NY 10003 USA

来源：

CELL | 2018年 / 174卷 / 04期

基金：

欧洲研究理事会; 比利时弗兰德研究基金会;

关键词：

QUALITY-CONTROL; R PACKAGE; GENE; RNA; NEURONS; PROTEIN; REVEALS; MOUSE; ENCODES; NETWORK;

D O I：

10.1016/j.cell.2018.05.057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The diversity of cell types and regulatory states in the brain, and how these change during aging, remains largely unknown. We present a single-cell transcriptome atlas of the entire adult Drosophila melanogaster brain sampled across its lifespan. Cell sorting. Our data show high granularity and identify a wide range of cell types. Gene network analyses using SCENIC revealed regulatory heterogeneity linked to energy consumption. During aging, RNA content declines exponentially without affecting neuronal identity in old brains. This single-cell brain atlas covers nearly all cells in the normal brain and provides the tools to study cellular diversity alongside other Drosophila and mammalian single-cell datasets in our unique single-cell analysis platform: SCope (http://scope.aertslab.org). These results, together with SCope, allow comprehensive exploration of all transcriptional states of an entire aging brain.

引用

页码：982 / +

页数：37

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