Setup for observing living cells using a commercial atomic force microscope

被引：10

作者：

Kasas, S

Wang, X

Hirling, H

Catsicas, S

Haeberli, C

Dietler, G

Thomson, N

机构：

[1] Univ Lausanne, Inst Biol Cellulaire & Morphol, CH-1005 Lausanne, Switzerland

[2] Univ Lausanne, Inst Physiol, CH-1005 Lausanne, Switzerland

[3] Univ Lausanne, Inst Phys Mat Condensee, CH-1015 Lausanne, Switzerland

[4] Ecole Polytech Fed Lausanne, Inst Genie Atom, CH-1015 Lausanne, Switzerland

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2000年 / 71卷 / 11期

关键词：

D O I：

10.1063/1.1318913

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

In this note, we present a setup which can be adapted to commercially available atomic force microscopes (Nanoscope II and III) to permit the observation of living cells in nearly physiological conditions. The setup permits one to heat the sample up to 40 degreesC, to exchange (without the use of the "O" ring) the imaging buffer while measuring, and to distinguish fluorescently labeled cell subpopulations. (C) 2000 American Institute of Physics. [S0034-6748(00)03411-0].

引用

页码：4338 / 4340

页数：3

共 12 条

[1] Design and construction of a heat stage for investigations of samples by atomic force microscopy above ambient temperatures [J].