Biotin and fluorescein labeling of biomolecules by active esters of 1-phenylpyrazolin-5-ones

被引：13

作者：

Kremsky, JN ^{[1
]}

Pluskal, M ^{[1
]}

Casey, S ^{[1
]}

PerryOKeefe, H ^{[1
]}

Kates, SA ^{[1
]}

Sinha, ND ^{[1
]}

机构：

[1] PERSEPT BIOSYST INC,FRAMINGHAM,MA 01701

来源：

TETRAHEDRON LETTERS | 1996年 / 37卷 / 25期

关键词：

biotin; fluorescein; oligonucleotides; peptide; PNA; probes; labeling;

D O I：

10.1016/0040-4039(96)00833-7

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The synthesis of 1-phenylpyrazolin-5-one carboxylate esters of biotin and fluorescein and their specific reactivity with amines under mild conditions to label various synthetic biopolymers such as oligonucleotides, peptides and PNA's is described. Copyright (C) 1996 Elsevier Science Ltd.

引用

页码：4313 / 4316

页数：4

共 13 条

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[5] APPLICATIONS OF COMBINATORIAL TECHNOLOGIES TO DRUG DISCOVERY .2. COMBINATORIAL ORGANIC-SYNTHESIS, LIBRARY SCREENING STRATEGIES, AND FUTURE-DIRECTIONS
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[10] POLYMER SUPPORT OLIGONUCLEOTIDE SYNTHESIS .18. USE OF BETA-CYANOETHYL-N,N-DIALKYLAMINO-/N-MORPHOLINO PHOSPHORAMIDITE OF DEOXYNUCLEOSIDES FOR THE SYNTHESIS OF DNA FRAGMENTS SIMPLIFYING DEPROTECTION AND ISOLATION OF THE FINAL PRODUCT
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