New scheme for electrochemical detection of DNA based on electrocatalytic oxidation of NADH

被引：10

作者：

de-los-SantosAlvarez, P ^{[1
]}

Rodríguez-Granda, P ^{[1
]}

Lobo-Castañón, MJ ^{[1
]}

Miranda-Ordieres, AJ ^{[1
]}

Tuñón-Blanco, P ^{[1
]}

机构：

[1] Univ Oviedo, Dept Quim Fis & Analit, Oviedo 33006, Spain

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2003年 / 5卷 / 03期

关键词：

DNA hybridisation; catalytic NADH oxidation; phenoxazines; phenothiazines; phenazines;

D O I：

10.1016/S1388-2481(03)00041-9

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A new electrocatalytic scheme for the detection of DNA is described. This scheme relies on the different interactions of singleand double-stranded oligonucleotides with the organic dyes methylene blue, thionin, azure A, azure B, toluidine blue O, brilliant cresyl blue and neutral red, which in addition can act as NADH catalysts. This catalytic activity can be used for amplifying differentiation between duplex and single-stranded DNA. Pyrolytic graphite electrodes modified with adsorbed dsDNA-organic dye complexes gave higher electrocatalytic NADH oxidation currents than those measured with electrodes modified with adsorbed ssDNA-organic dye. This difference is maximum for brilliant cresyl blue. These results suggest that the proposed scheme may be useful for the detection of DNA hybridisation and damage. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：267 / 271

页数：5

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