Temperature gradient focusing in miniaturized free-flow electrophoresis devices

被引：21

作者：

Becker, Marco ^{[1
]}

Mansouri, Abraham ^{[1
,2
]}

Beilein, Cornelia ^{[1
]}

Janasek, Dirk ^{[1
]}

机构：

[1] ISAS Inst Analyt Sci, Dortmund, Germany

[2] Univ Alberta, Dept Mech Engn, Edmonton, AB, Canada

来源：

ELECTROPHORESIS | 2009年 / 30卷 / 24期

关键词：

Free-flow electrophoresis; Lab-on-a-chip; Miniaturization; Temperature gradient focusing; CRUDE YEAST EXTRACT; ZONE ELECTROPHORESIS; SCALE-UP; ALCOHOL-DEHYDROGENASE; CONTINUOUS SEPARATION; MASS-SPECTROMETRY; CHIP; PURIFICATION; ISOTACHOPHORESIS; PROTEINS;

D O I：

10.1002/elps.200900359

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Temperature gradient focusing is a method to separate and focus any charged analytes even without accessible isoelectric point, and has been already widely used in CE. In this paper, we demonstrate the application of temperature gradient focusing to free-flow electrophoresis. Besides focusing and separation experiments of proteins, the stability of the temperature gradient under flow conditions and the temperature dependence of fluorescence dyes have also been investigated.

引用

页码：4206 / 4212

页数：7

共 34 条

[1] Simultaneous concentration and separation of enantiomers with chiral temperature gradient focusing [J].