The sgp gene modulates stress responses of Streptococcus mutans:: utilization of an antisense RNA strategy to investigate essential gene functions

被引：14

作者：

Sato, T ^{[1
]}

Wu, J ^{[1
]}

Kuramitsu, H ^{[1
]}

机构：

[1] SUNY Buffalo, Dept Oral Biol, Buffalo, NY 14214 USA

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 159卷 / 02期

关键词：

Streptococcus mutans; antisense; stress;

D O I：

10.1016/S0378-1097(97)00577-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The sgp gene from Streptococcus mutans has been previously isolated, characterized, and demonstrated to encode a G-protein. In order to investigate the function of this gene, a novel antisense RNA strategy was developed. Expression of sgp antisense RNA in Escherichia coli led to transient inhibition of growth. In addition, sgp antisense RNA expression in S. mutans resulted in decreased growth under environmental stress conditions (44 degrees C, acidic pH, and high osmolarity). Therefore, these results suggest that the sgp gene plays a role in modulating the stress responses of S. mutans. This approach could be applicable for investigating the function of essential genes in other organisms for which mutants are not available. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

引用

页码：241 / 245

页数：5

共 17 条

[1] A GTP-BINDING PROTEIN OF ESCHERICHIA-COLI HAS HOMOLOGY TO YEAST RAS PROTEINS [J].