Identification of inorganic crystal-specific sequences using phage display combinatorial library of short peptides: A feasibility study

被引：76

作者：

Gaskin, DJH ^{[1
]}

Starck, K ^{[1
]}

Vulfson, EN ^{[1
]}

机构：

[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 15期

基金：

英国生物技术与生命科学研究理事会;

关键词：

combinatorial library; crystal; peptide; phage display; recognition;

D O I：

10.1023/A:1005603117023

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The feasibility of using a combinatorial phage display library of decapeptides to identify ligands which can interact with the surface of a crystal was assessed using geological calcium carbonate as a model. Two relatively strong binding clones were identified by ELISA, sequenced and the encoded oligopeptides were prepared by solid phase synthesis and their properties compared with those of casein hydrolysate.

引用

页码：1211 / 1216

页数：6

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