Cloning and expression of rat GFAP cDNA in Escherichia coli

被引：8

作者：

Chekhonin, V. P.

Pavlov, K. A.

Shkoporov, A. N. ^{[1
]}

Yefimov, B. A.

Gurina, O. I.

Dmitrieva, T. B.

机构：

[1] VP Serbskii Ctr Social & Forens Psychiat, Dept Biol Psychiat, Lab Immunochem, Moscow, Russia

[2] Russian State Med Univ, Dept Basic & Clin Microbiol, Moscow 117437, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2007年 / 143卷 / 01期

关键词：

glial fibrillary acidic protein (GFAP); GFAP recombinant protein; escherichia coli; central nervous system;

D O I：

10.1007/s10517-007-0019-9

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

A cDNA fragment encoding GFAP was amplified by reverse transcription PCR from total mRNA isolated from primary culture of rat astrocytes and cloned for expression in Escherichia coli using pET-28a vector. High level of GFAP expression was confirmed by SDS-PAGE, while immunochemical identity was verified by immunoblotting. The constructed producer strain is a cheap source of GFAP and can be used for diagnostic purposes.

引用

页码：68 / 71

页数：4

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