1,4-dihydroxynonene mercapturic acid, the major end metabolite of exogenous 4-hydroxy-2-nonenal, is a physiological component of rat and human urine

被引：37

作者：

Alary, J ^{[1
]}

Debrauwer, L ^{[1
]}

Fernandez, Y ^{[1
]}

Cravedi, JP ^{[1
]}

Rao, D ^{[1
]}

Bories, G ^{[1
]}

机构：

[1] INRA, Lab Xenobiot, F-31931 Toulouse, France

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 1998年 / 11卷 / 02期

关键词：

D O I：

10.1021/tx970139w

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In the present study 1,4-dihydroxynonene mercapturic acid (DHN-MA), previously shown to be the major urinary metabolite of 4-hydroxy-2-nonenal (HNE) administered to the rat, was characterized and determined to be a normal constituent of rat and human urine. DHN-MA was excreted as a mixture of at least two stereoisomers as determined by ion trap LCMS/MS/MS after solid-phase extraction and HPLC purification. The 24-h urinary excretion of this compound was about 10 ng and 5 mu g for rat and human, respectively. This end metabolite of the lipid peroxidation product HNE could represent a specific and noninvasive biomarker.

引用

页码：130 / 135

页数：6

共 25 条

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