Inhibition of SARS-CoV replication by siRNA

被引:125
作者
Wu, CJ
Huang, HW
Liu, CY
Hong, CF
Chan, YL
机构
[1] Natl Taiwan Ocean Univ, Dept Food Sci, Chilung 202, Taiwan
[2] Natl Def Univ, Natl Def Med Ctr, Inst Prevent Med, Taipei, Taiwan
[3] Natl Def Univ, Natl Def Med Ctr, Inst Life Sci, Taipei, Taiwan
关键词
SARS-CoV; siRNA; spike protein; antiviral agent;
D O I
10.1016/j.antiviral.2004.09.005
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Serious outbreaks of severe acute respiratory syndrome (SARS), caused by the newly discovered coronavirus SARS-CoV occurred between late 2002 and early 2003 and there is an urgent need for effective antiviral agents. RNA interference in animals and post-transcriptional gene silencing plants is mediated by small double-stranded RNA molecules named small interfering RNA (siRNA). Recently, siRNA-induced RNA interference(RNAi) may provide a new approach to therapy for pathogenic viruses, e.g. HIV and HCV In this study, the silencing potential of seven synthetic siRNAs against SARS-CoV leader, TRS, 3'-UTR and Spike coding sequence have been applied to explore, the possibility for prevention of SARS-CoV infection. We demonstrate that siRNAs directed against Spike sequences and the 3'-UTR can inhibit the replication of SARS-CoV in Vero-E6 cells. and holds out promise for the development of an effective antiviral agent against SARS-CoV (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:45 / 48
页数:4
相关论文
共 12 条
[1]   Coronavirus main proteinase (3CLpro) structure:: Basis for design of anti-SARS drugs [J].
Anand, K ;
Ziebuhr, J ;
Wadhwani, P ;
Mesters, JR ;
Hilgenfeld, R .
SCIENCE, 2003, 300 (5626) :1763-1767
[2]   Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].
Elbashir, SM ;
Harborth, J ;
Lendeckel, W ;
Yalcin, A ;
Weber, K ;
Tuschl, T .
NATURE, 2001, 411 (6836) :494-498
[3]   Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate [J].
Elbashir, SM ;
Martinez, J ;
Patkaniowska, A ;
Lendeckel, W ;
Tuschl, T .
EMBO JOURNAL, 2001, 20 (23) :6877-6888
[4]   Modulation of HIV-1 replication by RNA interference [J].
Jacque, JM ;
Triques, K ;
Stevenson, M .
NATURE, 2002, 418 (6896) :435-438
[5]   Aminopeptidase N inhibitors and SARS [J].
Kontoyiannis, DP ;
Pasqualini, R ;
Arap, W .
LANCET, 2003, 361 (9368) :1558-1558
[6]   A novel coronavirus associated with severe acute respiratory syndrome [J].
Ksiazek, TG ;
Erdman, D ;
Goldsmith, CS ;
Zaki, SR ;
Peret, T ;
Emery, S ;
Tong, SX ;
Urbani, C ;
Comer, JA ;
Lim, W ;
Rollin, PE ;
Dowell, SF ;
Ling, AE ;
Humphrey, CD ;
Shieh, WJ ;
Guarner, J ;
Paddock, CD ;
Rota, P ;
Fields, B ;
DeRisi, J ;
Yang, JY ;
Cox, N ;
Hughes, JM ;
LeDuc, JW ;
Bellini, WJ ;
Anderson, LJ .
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1953-1966
[7]  
Lai M.M. C., 2001, FIELDS VIROLOGY, V4th, P1163
[8]   A major outbreak of severe acute respiratory syndrome in Hong Kong [J].
Lee, N ;
Hui, D ;
Wu, A ;
Chan, P ;
Cameron, P ;
Joynt, GM ;
Ahuja, A ;
Yung, MY ;
Leung, CB ;
To, KF ;
Lui, SF ;
Szeto, CC ;
Chung, S ;
Sung, JJY .
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1986-1994
[9]   The genome sequence of the SARS-associated coronavirus [J].
Marra, MA ;
Jones, SJM ;
Astell, CR ;
Holt, RA ;
Brooks-Wilson, A ;
Butterfield, YSN ;
Khattra, J ;
Asano, JK ;
Barber, SA ;
Chan, SY ;
Cloutier, A ;
Coughlin, SM ;
Freeman, D ;
Girn, N ;
Griffith, OL ;
Leach, SR ;
Mayo, M ;
McDonald, H ;
Montgomery, SB ;
Pandoh, PK ;
Petrescu, AS ;
Robertson, AG ;
Schein, JE ;
Siddiqui, A ;
Smailus, DE ;
Stott, JE ;
Yang, GS ;
Plummer, F ;
Andonov, A ;
Artsob, H ;
Bastien, N ;
Bernard, K ;
Booth, TF ;
Bowness, D ;
Czub, M ;
Drebot, M ;
Fernando, L ;
Flick, R ;
Garbutt, M ;
Gray, M ;
Grolla, A ;
Jones, S ;
Feldmann, H ;
Meyers, A ;
Kabani, A ;
Li, Y ;
Normand, S ;
Stroher, U ;
Tipples, GA ;
Tyler, S .
SCIENCE, 2003, 300 (5624) :1399-1404
[10]   Coronavirus as a possible cause of severe acute respiratory syndrome [J].
Peiris, JSM ;
Lai, ST ;
Poon, LLM ;
Guan, Y ;
Yam, LYC ;
Lim, W ;
Nicholls, J ;
Yee, WKS ;
Yan, WW ;
Cheung, MT ;
Cheng, VCC ;
Chan, KH ;
Tsang, DNC ;
Yung, RWH ;
Ng, TK ;
Yuen, KY .
LANCET, 2003, 361 (9366) :1319-1325