Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis

被引:540
作者
Hirai, Masami Yokota
Sugiyama, Kenjiro
Sawada, Yuji
Tohge, Takayuki
Obayashi, Takeshi
Suzuki, Akane
Araki, Ryoichi
Sakurai, Nozomu
Suzuki, Hideyuki
Aoki, Koh
Goda, Hideki
Nishizawa, Osamu Ishizaki
Shibata, Daisuke
Saito, Kazuki
机构
[1] RIKEN, Plant Sci Ctr, Tsuzuki Ku, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
[2] Kazusa DNA Res Inst, Chiba 2920818, Japan
[3] Chiba Univ, Grad Sch Pharmaceut Sci, Inage Ku, Chiba 2638522, Japan
[4] Kirin Brewery Co Ltd, Cent Labs Frontier Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
关键词
coexpression; functional genomics; transcriptomics;
D O I
10.1073/pnas.0611629104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Understanding plant metabolism as an integrated system is essential for metabolic engineering aimed at the effective production of compounds useful to human life and the global environment. The "omics" approach integrates transcriptome and metabolome data into a single data set and can lead to the identification of unknown genes and their regulatory networks involved in metabolic pathways of interest. one of the intriguing, although poorly described metabolic pathways in plants is the biosynthesis of glucosinolates (GSLs), a group of bioactive secondary products derived from amino acids that are found in the family Brassicaceae. Here we report the discovery of two R2R3-Myb transcription factors that positively control the biosynthesis of GSLs in Arabidopsis thaliana by an integrated omics approach. Combined transcriptome coexpression analysis of publicly available, condition-independent data and the condition-specific (i.e., sulfur-deficiency) data identified Myb28 and Myb29 as candidate transcription factor genes specifically involved in the regulation of aliphatic GSL production. Analysis of a knockout mutant and ectopic expression of the gene demonstrated that Myb28 is a positive regulator for basal-level production of aliphatic GSLs. Myb29 presumably plays an accessory function for methyl jasmonate-mediated induction of a set of aliphatic GSL biosynthetic genes. Overexpression of Myb28 in Arabidopsis-cultured suspension cells, which do not normally synthesize GSLs, resulted in the production of large amounts of GSLs, suggesting the possibility of efficient industrial production of GSLs by manipulation of these transcription factors. A working model for regulation of GSL production involving these genes, renamed Production of Methionine-Derived Glucosinolate (PMG) 1 and 2, are postulated.
引用
收藏
页码:6478 / 6483
页数:6
相关论文
共 43 条
[1]   Genome-wide Insertional mutagenesis of Arabidopsis thaliana [J].
Alonso, JM ;
Stepanova, AN ;
Leisse, TJ ;
Kim, CJ ;
Chen, HM ;
Shinn, P ;
Stevenson, DK ;
Zimmerman, J ;
Barajas, P ;
Cheuk, R ;
Gadrinab, C ;
Heller, C ;
Jeske, A ;
Koesema, E ;
Meyers, CC ;
Parker, H ;
Prednis, L ;
Ansari, Y ;
Choy, N ;
Deen, H ;
Geralt, M ;
Hazari, N ;
Hom, E ;
Karnes, M ;
Mulholland, C ;
Ndubaku, R ;
Schmidt, I ;
Guzman, P ;
Aguilar-Henonin, L ;
Schmid, M ;
Weigel, D ;
Carter, DE ;
Marchand, T ;
Risseeuw, E ;
Brogden, D ;
Zeko, A ;
Crosby, WL ;
Berry, CC ;
Ecker, JR .
SCIENCE, 2003, 301 (5633) :653-657
[2]  
An G, 1988, BINARY VECTORS
[3]  
AXELOS M, 1992, PLANT PHYSIOL BIOCH, V30, P123
[4]   Topological units of environmental signal processing in the transcriptional regulatory network of Escherichia coli [J].
Balázsi, G ;
Barabási, AL ;
Oltvai, ZN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (22) :7841-7846
[5]  
Batagelj V., 1998, Connections, V21, P47
[6]   The Arabidopsis ATR1 Myb transcription factor controls indolic glucosinolate homeostasis [J].
Celenza, JL ;
Quiel, JA ;
Smolen, GA ;
Merrikh, H ;
Silvestro, AR ;
Normanly, J ;
Bender, J .
PLANT PHYSIOLOGY, 2005, 137 (01) :253-262
[7]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[8]   AGRIS:: Arabidopsis Gene Regulatory Information Server, an information resource of Arabidopsis cis-regulatory elements and transcription factors -: art. no. 25 [J].
Davuluri, RV ;
Sun, H ;
Palaniswamy, SK ;
Matthews, N ;
Molina, C ;
Kurtz, M ;
Grotewold, E .
BMC BIOINFORMATICS, 2003, 4 (1)
[9]   Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens [J].
Fahey, JW ;
Zhang, YS ;
Talalay, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (19) :10367-10372
[10]   Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benzo[a]pyrene-induced stomach tumors [J].
Fahey, JW ;
Haristoy, X ;
Dolan, PM ;
Kensler, TW ;
Scholtus, I ;
Stephenson, KK ;
Talalay, P ;
Lozniewski, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (11) :7610-7615