Analysis of HLA-A24-restricted CMVpp65 peptide-specific CTL with HLA-A*2402-CMVpp65 tetramer

被引：10

作者：

Akiyama, Y

Kuzushima, K

Tsurumi, T

Yamaguchi, K

机构：

[1] Natl Canc Ctr, Res Inst, Chuo Ku, Div Growth Factor, Tokyo 104, Japan

[2] Shizuoka Canc Ctr, Inst Res, Div Immunotherapy, Shizuoka 4118777, Japan

[3] Aichi Canc Ctr, Res Inst, Div Immunol, Chikusa Ku, Nagoya, Aichi 4648681, Japan

[4] Aichi Canc Ctr, Res Inst, Div Virol, Chikusa Ku, Nagoya, Aichi 4648681, Japan

来源：

IMMUNOLOGY LETTERS | 2004年 / 95卷 / 02期

关键词：

CMV pp65 matrix protein; cytotoxic T cell; HLA-A24/CMVpp65; tetramer; INF-gamma production; anti-CMV antibody;

D O I：

10.1016/j.imlet.2004.07.010

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Developing precise and efficient methods of monitoring immune responses against cytomegalovirus (CMV) infection in immunocompromised transplantation patients is important. With the aim of optimizing the monitoring strategy, an HLA-A24-CMVpp65 tetramer-based analysis of CMVpp65 peptide-specific CTL lines was performed. Previously, the HLA-A24-restricted CTL epitope of CMVpp65 matrix protein was identified (QYDPVAALF aa 341-349). In the present study, CMVpp65 (aa 341-349) peptide- specific CTL lines were obtained from PBLs of 12 HLA-A24(+) healthy donors by two stimulations with peptide-pulsed dendritic cells (DC). Among 12 CTL lines, 9 showed HLA-A*2402-CMVpp65 tetramer staining, which was found to be strongly co-related to the amount of IFN-gamma produced by CMVpp65 peptider-estimulated CTL lines (r = 0.943, P < 0.001). These results suggested that HLA-A*2402-CMVpp65 tetramer staining was an efficient way to monitor immune responses against CMV infection in HLA-A24(+) immunocompromised hosts. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：199 / 205

页数：7

共 28 条

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