The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E-coli genomes

被引:312
作者
Johnson, Timothy J.
Kariyawasam, Subhashinie
Wannemuehler, Yvonne
Mangiamele, Paul
Johnson, Sara J.
Doetkott, Curt
Skyberg, Jerod A.
Lynne, Aaron M.
Johnson, James R.
Nolan, Lisa K.
机构
[1] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Coll Vet Med, Ames, IA 50011 USA
[2] N Dakota State Univ, Informat Technol Serv, Fargo, ND 58105 USA
[3] VA Med Ctr, Mucosal & Vaccine Res Ctr, Minneapolis, MN USA
[4] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA
关键词
D O I
10.1128/JB.01726-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Escherichia coli strains that cause disease outside the intestine are known as extraintestinal pathogenic E. coli (ExPEC) and include human uropathogenic E. coli (UPEC) and avian pathogenic E. coli (APEC). Regardless of host of origin, ExPEC strains share many traits. It has been suggested that these commonalities may enable APEC to cause disease in humans. Here, we begin to test the hypothesis that certain APEC strains possess potential to cause human urinary tract infection through virulence genotyping of 1,000 APEC and UPEC strains, generation of the first complete genomic sequence of an APEC (APEC O1:K1:H7) strain, and comparison of this genome to all available human ExPEC genomic sequences. The genomes of APEC O1 and three human UPEC strains were found to be remarkably similar, with only 4.5% of APEC O1's genome not found in other sequenced ExPEC genomes. Also, use of multilocus sequence typing showed that some of the sequenced human ExPEC strains were more like APEC O1 than other human ExPEC strains. This work provides evidence that at least some human and avian ExPEC strains are highly similar to one another, and it supports the possibility that a food-borne link between some APEC and UPEC strains exists. Future studies are necessary to assess the ability of APEC to overcome the hurdles necessary for such a food-borne transmission, and epidemiological studies are required to confirm that such a phenomenon actually occurs.
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页码:3228 / 3236
页数:9
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