Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy

被引：70

作者：

Huang, YS

Karashima, T

Yamamoto, M

Hamaguchii, H

机构：

[1] Univ Tokyo, Sch Sci, Dept Chem, Bunkyo Ku, Tokyo 113, Japan

[2] Univ Tokyo, Dept Biophys & Biochem, Tokyo 113, Japan

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2003年 / 34卷 / 01期

关键词：

time- and space-resolved Raman spectroscopy; living cell; mitosis; cell division; fission yeast;

D O I：

10.1002/jrs.960

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time- and space-resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi-channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time-dependent changes of major molecular components during the cell cycle were elucidated. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：1 / 3

页数：3

共 9 条

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