Screening of a specific monoclonal antibody against and detection of Listeria monocytogenes whole cells using a surface plasmon resonance biosensor

被引：15

作者：

Joung, Hyou-Arm

Shim, Won-Bo

Chung, Duck-Hwa

Ahn, Junhyoung

Chung, Bong Hyun

Choi, Ho-Suk

Ha, Sang-Do

Kim, Keun-Sung

Lee, Kyu-Ho

Kim, Cheol-Ho

Kim, Kwang-Yup

Kim, Min-Gon ^{[1
]}

机构：

[1] KRIBB, BioNanotechnol Res Ctr, Taejon 305806, South Korea

[2] Chungnam Natl Univ, Dept Chem Engn, Taejon 305764, South Korea

[3] Gyeongsang Natl Univ, Dept Food Sci & Technol, Jinju 660701, South Korea

[4] Chung Ang Univ, Dept Food Sci & Technol, Seoul 155756, South Korea

[5] Hankuk Univ Foreign Studies, Dept Environm Engn & Biotechnol, Yongin 449791, South Korea

[6] Sungkyunkwan Univ, Coll Nat Sci, Dept Biol Sci, Suwon 440746, South Korea

[7] Chungnam Natl Univ, Dept Food Sci & Technol, Cheongju 361763, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2007年 / 12卷 / 02期

关键词：

surface plasmon resonance (SPR); protein L; Listeria monocytogenes; antibody;

D O I：

10.1007/BF03028630

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, a specific monoclonal antibody against Listeria monocytogenes was screened using an SPR biosensor. Monoclonal antibodies were bound to protein L, after which the L. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine coupling method, and utilized repeatedly by regeneration. The monoclonal antibody, 'A18', was selected and employed for the high-sensitivity detection of L. monocytogenes. Under optimized conditions, 10(3) cells/mL or 50 cells were detected by the SPR biosensor. (c) KSBB.

引用

页码：80 / 85

页数：6

共 22 条

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