Characterization of thioredoxin y, a new type of thioredoxin identified in the genome of Chlamydomonas reinhardtii

被引：66

作者：

Lemaire, SD

Collin, V

Keryer, E

Quesada, A

Miginiac-Maslow, M

机构：

[1] Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR8618, F-91405 Orsay, France

[2] Fac Vet, Dept Bioquim & Biol Mol, Caceres 10071, Spain

来源：

FEBS LETTERS | 2003年 / 543卷 / 1-3期

关键词：

fructose-1,6-bisphosphate phosphatase; NADP-malate dehydrogenase; peroxiredoxin; thioredoxin; Chlamydomonas reinhardtii;

D O I：

10.1016/S0014-5793(03)00416-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequencing of the Arabidopsis genome revealed a multiplicity of thioredoxins (TRX), ubiquitous protein disulfide oxido-reductases. We have analyzed the TRX family in the genome of the unicellular green alga Chlamydomonas reinhardtii and identified eight different thioredoxins for which we have cloned and sequenced the corresponding cDNAs. One of these TRXs represents a new type that we named TRX y. This most probably chloroplastic TRX is highly conserved in photosynthetic organisms. The biochemical characterization of the recombinant protein shows that it exhibits a thermal stability profile and specificity toward target enzymes completely different from those of TRXs characterized so far. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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页码：87 / 92

页数：6

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