Measurements of DNA lengths remaining in a viral capsid after osmotically suppressed partial ejection

被引：58

作者：

Evilevitch, A ^{[1
]}

Gober, JW ^{[1
]}

Phillips, M ^{[1
]}

Knobler, CM ^{[1
]}

Gelbart, WM ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

来源：

BIOPHYSICAL JOURNAL | 2005年 / 88卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1529/biophysj.104.045088

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage-lambda was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids.

引用

页码：751 / 756

页数：6

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