Fast kinetic studies by fluorescence correlation spectroscopy of PNA-DNA interactions

被引：8

作者：

Aich, P ^{[1
]}

Nielsen, P ^{[1
]}

Rigler, R ^{[1
]}

机构：

[1] UNIV COPENHAGEN, PANUM INST, CTR BIOMOL RECOGNIT, DEPT BIOCHEM B, DK-2200 COPENHAGEN N, DENMARK

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1997年 / 16卷 / 5-6期

关键词：

D O I：

10.1080/07328319708002924

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics of the hybridization of fluorescently labeled Peptide nucleic acid (PNA) with its complementary single strand DNA templates and strand displacement from the same DNA duplexed with its 18-nucleotide(nt) long sequencing primer was measured. We used M13mp18 (+)ssDNA as template and the PNA molecule was 18-nt long with the identical sequence as to that of the sequencing primer of the DNA template. Kinetic studies show that hybridization was approximately 100-fold faster than the oligonucleotide-displacement at room temperature.

引用

页码：609 / 615

页数：7

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